Phenotypic and Genotypic Alterations of Durancin GL-Resistant Enterococcus durans Strains

文献类型： 外文期刊

第一作者： Du, Lihui

作者： Du, Lihui;Liu, Lingping;Ju, Xingrong;Yuan, Jian;Liu, Fang

作者机构：

期刊名称：FOODBORNE PATHOGENS AND DISEASE （ 影响因子：3.171； 五年影响因子：3.235 ）

ISSN： 1535-3141

年卷期： 2016 年 13 卷 6 期

页码：

收录情况： SCI

摘要： The emergence and spread of bacteriocin-resistant bacteria threaten the efficiency of bacteriocin usage as food preservatives. In this experiment, 19 selected Enterococcus durans strains acquired resistance after exposure to durancin GL, and the mutants had similar intermediate levels of resistance. One wild-type E. durans KLDS 6.0603 and its two resistant mutants, E. durans KLDS 6.0603-2 and E. durans KLDS 6.0603-3, were used to characterize phenotypic and genotypic differences. Approximately 100 mu g/mL of durancin GL can penetrate the cytoplasmic membrane of E. durans KLDS 6.0603, causing damage to bacterial cells, but cannot penetrate E. durans KLDS 6.0603-2 and KLDS 6.0603-3 membranes. Unsaturated fatty acid content in resistant strains was significantly increased compared with wild-type strains, indicating that the former has more fluidity of cell membrane than the latter. Decreased mannose phosphotransferase system gene expression (mptD) was observed in the two resistant strains. Results showed that the factors, including the increased unsaturated fatty acid and decreased mptD expression, could contribute to durancin GL resistance.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序