The RNAi lethal effect of a serine protease gene (TcSP3) on Tetranychus cinnabarinus and its safety evaluation

文献类型： 外文期刊

第一作者： Chen, Xing-Ru

作者： Chen, Xing-Ru;Rao, Teng-Yue;Liu, Guang-Ming;Chen, Yi-Ni;He, Lin;Shen, Guang-Mao;Chen, Xing-Ru;Rao, Teng-Yue;Liu, Guang-Ming;Chen, Yi-Ni;He, Lin;Shen, Guang-Mao;Luo, Yan-Jie

作者机构：

关键词： RNA Interference; risk assessment; side effect; spider mite; Neoseiulus sp

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：5.6； 五年影响因子：5.0 ）

ISSN： 0171-8177

年卷期： 2024 年

页码：

收录情况： SCI

摘要： RNA interference technology has been recognized in recent years as an ideal and specific new approach for pest control. In this study, a serine protease gene TcSP3 was found specifically expressed during the egg stage of Tetranychus cinnabarinus. Treatment of dsTcSP3 caused high mortality in egg and larvae stages of T. cinnabarinus, but a lower mortality rate after treating adult mites. Persistence analysis showed that dsTcSP3 degraded rapidly after application to cowpea leaves and was essentially undetectable after 60 h. It was also safe to Neoseiulus barkeri and caused essentially no death or deformity of N. barkeri. In conclusion, silencing of the serine protease gene TcSP3 successfully impeded the growth and development process of T. cinnabarinus with less impact on the environment and predatory mite (Neoseiulus barkeri), which can be used as a new target of RNAi for T. cinnabarinus.

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