New method for fabricating an alpha-fetoprotein affinity monolithic polymer array

文献类型: 外文期刊

第一作者: Li, Li

作者: Li, Li;Xia, Li-Ru;Wang, He-Ye;Bi, Xiao-Dong

作者机构:

关键词: photopolymerization;porous materials;sensors and actuators

期刊名称:JOURNAL OF APPLIED POLYMER SCIENCE ( 影响因子:3.125; 五年影响因子:2.754 )

ISSN: 0021-8995

年卷期: 2015 年 132 卷 16 期

页码:

收录情况: SCI

摘要: Bisphenol A based epoxy acrylate (BABEA), a commercial UV-curable material, was introduced as a crosslinker for the fabrication of an epoxy-functionalized monolithic polymer array through UV-initiated copolymerization with glycidyl methacrylate as the functional monomer and poly(ethylene glycol) 200 as the porogen. Scanning electron microscopy images showed that the monolithic poly(bisphenol A based epoxy acrylate-co-glycidyl methacrylate) [poly(BABEA-co-GMA)] exhibited a well-controlled skeletal and well-distributed porous structure. The alpha-fetoprotein (AFP) immunoaffinity monolithic polymer array prepared by the immobilization of AFP on epoxy-functionalized monolithic arrays was used as an immunosensor for chemiluminescent AFP detection. X-ray photoelectron spectroscopy results indicate that the AFP antibody was successfully immobilized on the monolithic poly(BABEA-co-GMA) array. With a noncompetitive immune-response format, the proposed AFP immunoaffinity array was demonstrated as a lowcost, flexible, homogeneous, and stable array for AFP detection. (C) 2014 Wiley Periodicals, Inc.

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