Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh)

文献类型： 外文期刊

第一作者： Liu Hongming

作者： Liu Hongming;Lou Xu;Yang Fan;Chen Dingbin;Zhu Jianchun;Xu Jianhong;Li Shunpeng;Hong Qing;Xu Jianhong;Ge Zhaojian

作者机构： Nanjing Agr Univ, Coll Life Sci, Key Lab Agr Environm Microbiol, Minist Agr, Nanjing 210095, Jiangsu, Peoples R China;Jiangsu Acad Agr Sci, Key Lab Agroprod Safety Risk Evaluat, Jiangsu Ctr

关键词： fenoxaprop-P-ethyl;microbial degradation;Rhodococcus ruber JPL-2;FE-hydrolyzing carboxylesterase gene

期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 2020影响因子：2.476； 五年影响因子：3.391 ）

ISSN： 1517-8382

年卷期： 2015 年 46 卷 2 期

页码：

收录情况： SCI

摘要： The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L-1 FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofopmethy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.

分类号：

  • 相关文献

[1]Identification of Reference Genes for Studying Herbicide Resistance Mechanisms in Japanese Foxtail (Alopecurus japonicus). Xu, Hongle,Li, Jun,Wu, Xibao,Wang, Lingyue,Dong, Liyao,Xu, Hongle,Wu, Renhai,Su, Wangcang.

[2]The role of cytochrome P450 monooxygenase in the different responses to fenoxaprop-P-ethyl in annual bluegrass (Poa annua L.) and short awned foxtail (Alopecurus aequalis Sobol.). Wang, Hongchun,Li, Jun,Dong, Liyao,Wang, Hongchun,Li, Jun,Dong, Liyao,Wang, Hongchun,Lou, Yuanlai,Lv, Bo. 2013

[3]Microbial degradation of endosulfan in contaminated soil with the elution of surfactants. Deng, Fei,Xiong, Bailian,Zhang, Jinzhong,Xiong, Bailian,Chen, Benshou,Zheng, Guocan,Zhang, Jinzhong.

[4]Shifts in indigenous microbial communities during the anaerobic degradation of pentachlorophenol in upland and paddy soils from southern China. Chen, Yating,Chen, Yating,Tao, Liang,Wu, Ke,Wang, Yongkui,Chen, Yating,Wu, Ke. 2016

[5]Advances in Microbial Degradation of Organophophates and Organophosphorus Hydrolase. Wang, Jinbin,Tan, Furong,Jiang, Lingxi,Wang, Ligang,Yang, Qi,Tang, Xueming. 2010

[6]Characterization and application of a newly isolated pyrene-degrading bacterium, Pseudomonas monteilii. Ping, Lifeng,Yuan, Xiaoli,Shan, Shengdao,Ping, Lifeng,Zhang, Chunrong,Cui, He,Cui, Juntao. 2017

作者其他论文 更多>>

微信小程序