Capturing Protein-Protein Interactions with Acidic Amino Acids Reactive Cross-Linkers

文献类型： 外文期刊

第一作者： Liao, Qing-Qing

作者： Liao, Qing-Qing;Shu, Xin;Sun, Wei;Zhang, Long;Yang, Bing;Liao, Qing-Qing;Xie, Feng;Zhang, Zhengkui;Dai, Tong;Wang, Shuai;Zhou, Fangfang;Mandapaka, Hyma;Wu, Haifan;Zhao, Jinghua;Lin, Jinzhong;Jiang, Hong;Li, Shu-Wei;Coin, Irene;Yang, Fan;Peng, Jinrong;Li, Kui

作者机构：

关键词： acidic amino acid cross-linking; chemical cross-linker; cross-linking mass spectrometry; p53

期刊名称：SMALL （ 影响因子：13.3； 五年影响因子：13.2 ）

ISSN： 1613-6810

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Acidic residues (Asp and Glu) have a high prevalence on protein surfaces, but cross-linking reactions targeting these residues are limited. Existing methods either require high-concentration coupling reagents or have low structural compatibility. Here a previously reported "plant-and-cast" strategy is extended to develop heterobifunctional cross-linkers. These cross-linkers first react rapidly with Lys sidechains and then react with Asp and Glu sidechains, in a proximity-enhanced fashion. The cross-linking reaction proceeds at neutral pH and room temperature without coupling reagents. The efficiency and robustness of cross-linking using model proteins, ranging from small monomeric proteins to large protein complexes are demonstrated. Importantly, it is shown that this type of cross-linkers are efficient at identifying protein-protein interactions involving acidic domains. The Cross-linking mass spectrometry (XL-MS) study with p53 identified 87 putative binders of the C-terminal domain of p53. Among them, SARNP, ZRAB2, and WBP11 are shown to regulate the expression and alternative splicing of p53 target genes. Thus, these carboxylate-reactive cross-linkers will further expand the power of XL-MS in the analysis of protein structures and protein-protein interactions. Acidic amino acids Asp/Glu widely distribute on protein surfaces and are involved in protein electrostatic interaction. In this work, stable and highly efficient acidic amino acids reactive cross-linkers are synthesized and applied to capture and identify p53 c-terminal domain-mediated direct protein interactions.image

分类号：

  • 相关文献
作者其他论文 更多>>
  • Impact of residual antibiotics on microbial decomposition of livestock manures in Eutric Regosol: Implications for sustainable nutrient recycling and soil carbon sequestration

    作者：Fang, Linfa;Lakshmanan, Prakash;Su, Xiaoxuan;Shi, Yujia;Chen, Zheng;Zhang, Yu;Xiao, Ran;Chen, Xinping;Lakshmanan, Prakash;Wu, Junxi;Lakshmanan, Prakash;Lakshmanan, Prakash;Sun, Wei

    关键词：Residual antibiotics; Livestock manure decomposition; Microbial community; Co -occurrence network; Enzyme activities

  • Extreme drought impacts have been underestimated in grasslands and shrublands globally

    作者：Smith, Melinda D.;Knapp, Alan K.;Ohlert, Timothy;Smith, Melinda D.;Knapp, Alan K.;Wilkins, Kate D.;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Wilfahrt, Peter;Anderson, Maggie;Borer, Elizabeth T.;Isbell, Forest;Seabloom, Eric W.;Collins, Scott L.;Sala, Osvaldo E.;Jordan, Samuel;Dukes, Jeffrey S.;Phillips, Richard P.;Beier, Claus;Yahdjian, Laura;Biancari, Lucio;Bondaruk, Viviana Florencia;Onatibia, Gaston R.;Pineiro-Guerra, Juan Manuel;Gherardi, Laureano A.;Larsen, Klaus Steenberg;Fraser, Lauchlan H.;Jentsch, Anke;Niu, Yujie;Loik, Michael E.;Maestre, Fernando T.;Valdecantos, Alejandro;Maestre, Fernando T.;Gozalo, Beatriz;Maturano-Ruiz, Adrian;Valdecantos, Alejandro;Power, Sally A.;Chieppa, Jeff;Nielsen, Uffe N.;Yu, Qiang;Felton, Andrew J.;Munson, Seth M.;Luo, Yiqi;Bauerle, Taryn;Abdoli, Hamed;Abedi, Mehdi;Bahalkeh, Khadijeh;Alados, Concepcion L.;Castillo-Garcia, Miguel;Pueyo, Yolanda;Alberti, Juan;Bruschetti, Carlos Martin;Cardoni, Augusto;Daleo, Pedro;Iribarne, Oscar;Alon, Moshe;Cohen, Ofer;Sternberg, Marcelo;An, Hui;Anacker, Brian;Auge, Harald;Auge, Harald;Eisenhauer, Nico;Hoss, Daniela;Roscher, Christiane;Sunnemann, Marie;Bachle, Seton;Bachle, Seton;Bahn, Michael;Ingrisch, Johannes;Batbaatar, Amgaa;Cahill, James F., Jr.;Batbaatar, Amgaa;Bork, Edward W.;Carlyle, Cameron N.;Behn, Kai;Beil, Ilka;Kreyling, Juergen;Malyshev, Andrey V.;Blindow, Irmgard;Dahlke, Sven;Byrne, Kerry M.;Calvo, Dianela A.;Leder, Cintia V.;Peter, Guadalupe;Carbognani, Michele;Forte, T'ai G. W.;Petraglia, Alessandro;Chang, Scott X.;Cianciaruso, Marcus V.;Guidoni-Martins, Karlo G.;Nobre, Paola;Cordeiro, Amanda L.;Cusack, Daniela F.;Dietterich, Lee H.;D'Antonio, Carla M.;Dietterich, Lee H.;Doherty, Tim S.;Greenville, Aaron C.;Wardle, Glenda M.;Dubbert, Maren;Ebeling, Anne;Eisenhauer, Nico;Fischer, Felicia M.;Hoss, Daniela;Sunnemann, Marie;Hoss, Daniela;Pillar, Valerio D.;Fischer, Felicia M.;Gebauer, Tobias;Anacker, Brian;Haugum, Siri Vatso;Vandvik, Vigdis;Hautier, Yann;Hefting, Mariet;Henry, Hugh A. L.;Johnson, Yari;Kelly, Eugene F.;Kimmel, Kaitlin;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kubert, Angelika;Werner, Christiane;Lamb, Eric G.;Larson, Julie;O'Connor, Rory C.;Lawson, Jason;Plowes, Robert M.;Linstadter, Anja;Orban, Ildiko;Liu, Jielin;Liu, Shirong;Longo, Grisel;Loydi, Alejandro;Rodriguez, Dana Aylen;Scarfo, Maria Cecilia;Luan, Junwei;Wang, Yi;Luan, Junwei;Wang, Yi;Lubbe, Frederick Curtis;Wiehl, Georg;Mackie-Haas, Kathleen;Stampfi, Andreas;Merchant, Thomas;Metcalfe, Daniel B.;Mori, Akira S.;Mori, Akira S.;Mudongo, Edwin;Newman, Gregory S.;Souza, Lara;Nimmo, Dale;Ogaya, Roma;Penuelas, Josep;Osborne, Brooke;Otfinowski, Rafael;Partel, Meelis;Pineiro-Guerra, Juan Manuel;Peri, Pablo L.;Picon-Cochard, Catherine;Pineiro-Guerra, Juan Manuel;Ploughe, Laura W.;Portales-Reyes, Cristy;Reed, Sasha C.;Ritchie, Euan G.;Roscher, Christiane;Sanchez, Ana M.;Santo, Braulio A.;Shi, Baoku;Sun, Wei;Souza, Lara;Standish, Rachel J.;Zeiter, Michaela;Stampfi, Andreas;Zeiter, Michaela;Standish, Rachel J.;Tedder, Michelle;Thorvaldsen, Pal;Tian, Dashuan;Zhu, Juntao;Zong, Ning;Tielborger, Katja;van den Brink, Liesbeth;Vankoughnett, Mathew R.;Velle, Liv Guri;Wang, Changhui;Wei, Cunzheng;Williams, Jennifer L.;Wolf, Amelia A.;Zhang, Fawei;Zuo, Xiaoan;Zuo, Xiaoan;Gebauer, Tobias;Ploughe, Laura W.

    关键词：climate extreme; Drought-Net; International Drought Experiment; productivity

  • Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system

    作者：Su, Xiaoyun;Zhang, Honglian;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Wang, Shuai;Ji, Wangli;Zhang, Wei;Hakulinen, Nina

    关键词：Manganese peroxidase; Deoxynivalenol; ScFv; Glutathione; Mycotoxin detoxification

  • miR-329b-5p Affects Sheep Intestinal Epithelial Cells against Escherichia coli F17 Infection

    作者：Xu, Yeling;Chen, Weihao;Sun, Wei;Yang, Huiguo;Song, Zhenghai;Wang, Yeqing;Su, Rui;Mwacharo, Joram M.;Lv, Xiaoyang;Sun, Wei;Lv, Xiaoyang;Sun, Wei

    关键词：Escherichia coli F17; micro RNA; sheep diarrhea; intestinal epithelial cells

  • Morphological and molecular analyses reveal two new species of Grifola (Polyporales) from Yunnan, China

    作者：Tang, Song-Ming;Chen, De-Chao;Wang, Shuai;Wu, Xiao-Qu;Ao, Cheng-Ce;Li, Er-Xian;Luo, Hong-Mei;Li, Shu-Hong;Chen, De-Chao;Wang, Shuai;Wu, Xiao-Qu;Ao, Cheng-Ce

    关键词：2 new species; morphology; multi-gene phylogeny; Southeast Asia; taxonomy; Yunnan

  • A telomere-to-telomere genome assembly of Hongyingzi, a sorghum cultivar used for Chinese Baijiu production

    作者：Ding, Yanqing;Xu, Jianxia;Li, Wenzhen;Cheng, Bin;Cao, Ning;Gao, Xu;Zhang, Liyi;Wang, Yilin;Jiang, Feng;Zhang, Qiaoling;Yang, Longping;Yang, Fan;Zhao, Zhenyu;Zhang, Xin;Zou, Guihua

    关键词：Sorghum; Telomere-to-telomere assembly; Hongyingzi (HYZ); Chinese Baijiu-making cultivar

  • Transcriptome differential expression analysis of defoliation of two different lemon varieties

    作者：Dong, Meichao;Gao, Junyan;Yang, Fan;Wang, Shaohua;Long, Chunrui;Fu, Xiaomeng;Liu, Hongming;Guo, Lina;Zhou, Dongguo;Yin, Tuo;Zhang, Hanyao

    关键词：Lemon; Defoliation; Transcriptome; WGCNA; Differentially expressed genes

微信小程序