Target gene mutation and enhanced metabolism confer fomesafen resistance in an Amaranthus retroflexus L. population from China

文献类型： 外文期刊

第一作者： Cao, Yi

作者： Cao, Yi;Huang, Hongjuan;Wei, Shouhui;Lan, Yuning;Li, Wenyu;Sun, Ying;Wang, Ruolin;Huang, Zhaofeng

作者机构：

关键词： Fomesafen; Mutation; Non-target-site resistance mechanisms; Amaranthus retroflexus L; Metabolism

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.966； 五年影响因子：4.591 ）

ISSN： 0048-3575

年卷期： 2022 年 188 卷

页码：

收录情况： SCI

摘要： Amaranthus retroflexus L., a troublesome annual dicotyledonous weed species, is highly competitive with soybean (Glycine max L.). A single-dose herbicide-resistance screening assay identified an A. retroflexus population with suspected resistance to fomesafen. Whole-plant dose-response assays demonstrated that the resistant population (2492) was resistant to protoporphyrinogen oxidase (PPO)-inhibiting herbicides (50.6-fold fomesafen resistance and > 8.1-fold lactofen resistance) compared to a susceptible (S) population. PPX2 gene sequence analysis showed an Arg128Gly amino acid substitution in the 2492 population. Moreover, pretreatment of malathion and the fomesafen metabolic assays through HPLC-MS demonstrated enhanced fomesafen metabolism in the 2492 population. Additionally, the 2492 population was 10.4-fold more resistant to the ALS-inhibiting herbicide imazethapyr and 16.8-fold more resistant to thifensulfuron-methyl than the S population. ALS gene sequence analysis showed an Ala205Val amino acid substitution in the 2492 population. This population of A. retroflexus has coexisting target-site resistance and non-target-site mechanisms for resistance to fomesafen. Multiple her-bicide resistance may mean it is necessary to adjust weed management strategies to better control the resistant population.

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