Determination of Deoxynivalenol-3-Glucoside in Cereals by Hydrophilic Interaction Chromatography with Ultraviolet Detection

文献类型: 外文期刊

第一作者: Geng, Zhiming

作者: Geng, Zhiming;Wang, Daoying;Liu, Fang;Zhu, Yongzhi;Zhang, Muhan;Yang, Dan;Zhou, Miaoping;Zhang, Pingping

作者机构:

关键词: Deoxynivalenol-3-glucoside;Cereal;Hydrophilic interaction chromatography;Ultraviolet detection

期刊名称:FOOD ANALYTICAL METHODS ( 影响因子:3.366; 五年影响因子:3.07 )

ISSN: 1936-9751

年卷期: 2014 年 7 卷 5 期

页码:

收录情况: SCI

摘要: A new hydrophilic interaction liquid chromatography (HILIC) method was developed for the determination of deoxynivalenol-3-glucoside (D3G) in cereals. D3G was extracted with water and cleaned with an immunoaffinity (IA) column, followed by chromatographic separation on a Syncronis HILIC column with acetonitrile-water (90:10, v/v) as the mobile phase, and detected at 220 nm by UV detection. The calibration curve was found to be linear in the range of 0.1-2.0 mu g/ml. Limit of detection and limit of quantification were 8 and 25 mu g/kg, respectively. The method was applied to the determination of D3G in 14 cereal samples, which were analyzed as well as by LC-MS/MS. The results indicate that the method can be considered as an alternative to LC-MS/MS and could be adopted by nonprofessional analytical labs.

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