FgIlv5 is required for branched-chain amino acid biosynthesis and full virulence in Fusarium grammearum

文献类型： 外文期刊

第一作者： Liu, Xin

作者： Liu, Xin;Wang, Jian;Xu, Jianhong;Shi, Jianrong

作者机构：

期刊名称：MICROBIOLOGY-SGM （ 影响因子：2.777； 五年影响因子：2.871 ）

ISSN： 1350-0872

年卷期： 2014 年 160 卷

页码：

收录情况： SCI

摘要： In this study, we characterized FgIlv5, a homologue of the Saccharomyces cerevisiae keto-acid reductoisomerase (KARI) from the important wheat head scab fungus Fusarium graminearum. KARI is a key enzyme in the branched-chain amino acid (BCAA, including leucine, isoleucine and valine) biosynthetic pathway that exists in a variety of organisms from bacteria to fungi and higher plants, but not in mammals. The FgILV5 deletion mutant Delta FgIlv5-4 failed to grow when the culture medium was nutritionally limited for BCAAs. When grown on potato-dextrose agar plates, Delta FgIlv5-4 exhibited a significant decrease in aerial hyphae formation and red pigmentation. Conidia formation was also blocked in Delta FgIlv5-4. Exogenous addition of 1 mM isoleucine and valine was able to rescue the defects of mycelial growth and conidial morphogenesis. Cellular stress assays showed that Delta FgIlv5-4 was more sensitive to osmotic and oxidative stresses than the wild-type strain. In addition, virulence of Delta FgIlv5-4 was dramatically reduced on wheat heads, and a low level of deoxynivalenol production was detected in Delta FgIlv5-4 in wheat kernels. The results of this study indicate that FgIlv5 is involved in valine and isoleucine biosynthesis and is required for full virulence in F. graminearum.

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