Contribution of Eukaryotic-Type Serine/Threonine Kinase to Stress Response and Virulence of Streptococcus suis

文献类型： 外文期刊

第一作者： Zhu, Haodan

作者： Zhu, Haodan;Zhou, Junming;Ni, Yanxiu;Yu, Zhengyu;Mao, Aihua;Hu, Yiyi;Wang, Wei;Zhang, Xuehan;Wen, Libin;Li, Bin;Wang, Xiaomin;Yu, Yang;Lv, Lixin;Guo, Rongli;He, Kongwang;Zhu, Haodan;Zhou, Junming;Ni, Yanxiu;Yu, Zhengyu;Mao, Aihua;Hu, Yiyi;Wang, Wei;Zhang, Xuehan;Wen, Libin;Li, Bin;Wang, Xiaomin;Yu, Yang;Lv, Lixin;Guo, Rongli;He, Kongwang;Lu, Chengping

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 3 期

页码：

收录情况： SCI

摘要： Streptococcus suis serotype 2 (SS2) is an important swine and human pathogen responsible for septicemia and meningitis. The bacterial homologues of eukaryotic-type serine/threonine kinases (ESTKs) have been reported to play critical roles in various cellular processes. To investigate the role of STK in SS2, an isogenic stk mutant strain (Dstk) and a complemented strain (CDstk) were constructed. The Dstk showed a significant decrease in adherence to HEp-2 cells, compared with the wild-type strain, and a reduced survival ratio in whole blood. In addition, the Dstk exhibited a notable reduced tolerance of environmental stresses including high temperature, acidic pH, oxidative stress, and high osmolarity. More importantly, the Dstk was attenuated in both the CD1 mouse and piglet models of infection. The results of quantitative reverse transcription-PCR (qRT-PCR) analysis indicated that the expressions of a few genes involving in adherence, stress response and virulence were clearly decreased in the Dstk mutant strain. Our data suggest that SsSTK is required for virulence and stress response in SS2.

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