Identification and Genetic Division of Fusarium graminearum and Fusarium asiaticum by Species-Specific SCAR Markers

文献类型： 外文期刊

第一作者： Zhang, Xu

作者： Zhang, Xu;Ma, Hong-xiang;Zhou, Yong-jin;Xing, Jin-cheng;Chen, Jian-hua;Yu, Gui-hong;Sun, Xiao-bo;Wang, Lei;Zhou, Yong-jin

作者机构：

关键词： chemotype;Fusarium;sequence-characterized amplified region;sequence-related amplified polymorphism;species

期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.789； 五年影响因子：1.574 ）

ISSN： 0931-1785

年卷期： 2014 年 162 卷 2 期

页码：

收录情况： SCI

摘要： Fusarium head blight (FHB), also called scab, is a devastating and insidious disease of cereals including wheat (Triticum spp.) and barley (Hordeum vulgare L.) worldwide. Apart from direct yield losses, the most serious concern about FHB is the contamination of the crop with mycotoxins, which pose a health risk to human and livestock. Recent research reported that phylogenetic species F.asiaticum (Fa) and F.graminearum (Fg) were the major causal agents of FHB from infected wheat heads in China. To investigate the population structure of Fusarium species in China by species-specific as well as the chemotype-specific markers, sequence-related amplified polymorphism (SRAP) markers were screened on representative isolates of F.asiaticum-NIV, F.asiaticum- 3ADON and F.graminearum-15ADON to find amplification products characteristic of either species or chemotypes. Selected amplified fragments were cloned and sequenced so that sequence-characterized amplified region (SCAR) primer pairs could be developed which permit specific detection of Fusarium species using conventional PCR. Primer pairs SCAR-Fa1 and SCAR-Fg1 were confirmed to be able to amplify specific products only in F.asiaticum and F.graminearum isolates, respectively. These species-specific primers were applied to determine genetic division of F.asiaticum and F.graminearum isolates collected in Yangtze-Huaihe valley. The results indicated that F.asiaticum was the predominant species causing FHB in this wheat production area. It is the first report that SRAP markers were adapted for species characterization in Fusarium isolates.

分类号：

  • 相关文献

[1]Development of a sequence-characterized amplified region marker for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa Kuhn. Gao, L.,Liu, T. G.,Chen, W. Q.. 2009

[2]Chemotypical variabiulity of leaf oils in Elephantopus scaber from 12 locations in China. Wang, L,Jian, SG,Nan, P,Liu, JM,Zhong, Y. 2005

[3]The expression of pectin methylesterase in onion flower buds is associated with the dominant male-fertility restoration allele. Huo, Yumeng,Miao, Jun,Liu, Bingjiang,Yang, Yanyan,Zhang, Yihui,He, Qiwei,Wu, Xiong,Huo, Yumeng,Miao, Jun,Liu, Bingjiang,Yang, Yanyan,Zhang, Yihui,He, Qiwei,Wu, Xiong,Huo, Yumeng,Miao, Jun,Liu, Bingjiang,Yang, Yanyan,Zhang, Yihui,He, Qiwei,Wu, Xiong,Tahara, Yasuki,Meng, Qingwei,Kitano, Hidemi. 2012

[4]Genetic linkage map construction for kenaf using SRAP, ISSR and RAPD markers. Chen, Mei-xia,Wei, Cheng-lin,Qi, Jian-min,Tao, Ai-fen,Wu, Wei-ren,Chen, Xing-bo,Su, Jian-guang,Li, Ai-Qing. 2011

[5]Evaluation of genetic diversity and relationships in orchardgrass (Dactylis glomerata L.) germplasm based on SRAP markers. Zhang, Xin-Quan,Zeng, Bing,Lan, Ying,Yang, Wu-Yun.

[6]Identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (Brassica oleracea L. var. acephala). Wang, Y. S.,Liu, Z. Y.,Zhang, Y.,Yang, X. F.,Feng, H.,Wang, Y. S.,Li, Y. F.. 2013

[7]Identification of sequence-related amplified polymorphism and insertion-deletion markers linked to the male fertility restorer gene of pol-like CMS06J45 in heading Chinese cabbage (Brassica rapa subsp pekinensis). Xu, X. Y.,Zhang, Y.,Zhang, L. G.,Xu, X. Y.,Fang, Z. Y.. 2014

[8]Molecular mapping of MS-cd1 gene in Chinese kale. Wu, Jian,Zhang, Hui,Fang, Zhi-yuan,Wang, Xiao-wu,Zhang, Xin-mei,Guo, Ai-guang,Ma, Yuan. 2010

[9]Sequence-related amplified polymorphism, an effective molecular approach for studying genetic variation in Fasciola spp. of human and animal health significance. Li, Qiao-Yan,Lin, Rui-Qing,Song, Hui-Qun,Zhu, Xing-Quan,Dong, Shi-Juan,Zhang, Wei-Yu,Wang, Chun-Ren,Qian, De-Xing. 2009

[10]Analysis of Genetic Diversity in Chrysopogon aciculatus Using Intersimple Sequence Repeat and Sequence-related Amplified Polymorphism Markers. Zhang, Xinyi,Liao, Li,Wang, Zhiyong,Bai, Changjun,Liu, Jianxiu.

[11]Molecular mapping of a new red mutant gene (R-s) in upland cotton. Yin, J. M.,Chen, X. S.,Xiao, S. H.,Xu, N. Y.,Die, J. C.,Liu, J. G.,Wu, Q. J..

[12]Genetic linkage map construction for white jute (Corchorus capsularis L.) using SRAP, ISSR and RAPD markers. Chen, Yanping,Zhang, Liwu,Qi, Jianmin,Tao, Aifen,Xu, Jiantang,Lin, Lihui,Fan, Pingping,Chen, Yanping,Chen, Hui.

[13]Comparative Study of Phenolic Compounds and Antioxidant Activity in Different Species of Cherries. Liu, Yun,Zhong, Fei,Tian, Rongrong,Li, Tianhong,Liu, Xinyan,Zhang, Kaichun,Zhang, Xiaoming. 2011

[14]Western honeybee drones and workers (Apis mellifera ligustica) have different olfactory mechanisms than eastern honeybees (Apis cerana cerana). Woltedji, Dereje,Song, Feifei,Zhang, Lan,Gala, Alemayehu,Han, Bin,Feng, Mao,Fang, Yu,Li, Jianke.

[15]Refining a biotic ligand model for nickel toxicity to barley root elongation in solution culture. Li, Bo,Ma, Yibing,Zhang, Xuan,Wang, Xuedong,Zhang, Xuan,Wang, Xuedong.

[16]Proteomic analysis of cow, yak, buffalo, goat and camel milk whey proteins: Quantitative differential expression patterns. Yang, Yongxin,Bu, Dengpan,Zhao, Xiaowei,Sun, Peng,Wang, Jiaqi,Zhou, Lingyun,Yang, Yongxin,Zhao, Xiaowei.

[17]Responses of pepper to waterlogging stress. Dai, X. Z.,Zhang, Z. Q.,Zou, X. X.,Ou, L. J.,Ou, L. J.. 2011

[18]Prevalence of Coccidiosis in Domestic Rabbits (Oryctolagus cuniculus) in Northwest China. Qiao, J.,Meng, Q. L.,Chen, C. F.,Wang, J. W.,Wang, W. S.,Zhang, Z. C.,Cai, K. J.,Yang, L. H.,Qiao, J.,Cai, X. P.,Tian, G. F.. 2012

[19]Rumen ciliated protozoan fauna of the yak (Bos grunniens) in China with the description of Entodinium monuo n. sp.. Guirong,Su, NR,Hua, ZX,Zhu, S,Imai, SC.

[20]Arsenic Distribution, Species, and Its Effect on Maize Growth Treated with Arsenate. Ci Xiao-ke,Liu Hua-lin,Hao Yu-bo,Zhang Ji-wang,Liu Peng,Dong Shu-ting,Liu Hua-lin,Ci Xiao-ke. 2012

作者其他论文 更多>>

微信小程序