UV-Initiated Copolymerization Route for Facile Fabrication of Epoxy-Functionalized Micro-Zone Plates

文献类型: 外文期刊

第一作者: Li, Li

作者: Li, Li;Feng, Min;Zhu, Jia-Ting;Bi, Xiao-Dong

作者机构:

关键词: applications;biomaterials;copolymers

期刊名称:JOURNAL OF APPLIED POLYMER SCIENCE ( 影响因子:3.125; 五年影响因子:2.754 )

ISSN: 0021-8995

年卷期: 2014 年 131 卷 2 期

页码:

收录情况: SCI

摘要: Bisphenol A-based epoxy acrylate (BABEA), a commercial ultraviolet (UV)-curable material, was introduced as a new manufacturing material for facile fabrication of epoxy-functionalized micro-zone plates through UV-initiated copolymerization using glycidyl methacrylate (GMA) as the functional monomer. The poly (BABEA-co-GMA) was highly transparent in visible range while highly opaque when the wavelength is less than 295 nm, and of high replication fidelity. X-ray photoelectron spectroscope (XPS) results indicated the existence of epoxy groups on the surface of the poly (BABEA-co-GMA), which allowed for binding protein through an epoxy-amino group reaction. A fabrication procedure was proposed for manufacturing BABEA based epoxy-functionalized micro-zone plates. The fabrication procedure was very simple; obviating the need of micromachining equipments, wet etching or imprinting techniques. To evaluate the BABEA-based epoxy-functionalized micro-zone plates, alpha-fetoprotein (AFP) was immobilized onto the capture zone for chemiluminescent (CL) detection in a noncompetitive immune response format. The proposed AFP immunoaffinity micro-zone plate was demonstrated as a low cost, flexible, homogeneous, and stable assay for alpha-fetoprotein (AFP). (C) 2013 Wiley Periodicals, Inc.

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