Cloning and expression analysis of PpSUT2 encoding a sucrose transporter in pear

文献类型： 外文期刊

第一作者： Tang, J.

作者： Tang, J.;Lin, J.;Zhang, B. L.;Wang, Z. H.;Li, X. G.;Chang, Y. H.;Tang, J.

作者机构：

关键词： Sucrose transporter;PpSUT2;Subcellular localization;Real-time fluorescent quantitative polymerase chain reaction;Prokaryotic expression

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 4 期

页码：

收录情况： SCI

摘要： A 1794-bp cDNA fragment was amplified from mRNA isolated from pear (Pyrus pyrifolia NaKai. Cuiguan) leaves by using primers based on the sequences generated during the analysis of the pear transcriptome. The 597-amino acid sequence encoded by the cDNA was compared with the sequences in GenBank, and it was found to be similar to that of members of the sucrose-proton cotransporter family. The hydrophobic protein, which was predicted to have 11 transmembrane domains, was designated as PpSUT2. Real-time fluorescent quantitative polymerase chain reaction analysis indicated the accumulation of PpSUT2 mRNA throughout the plant, with the highest levels in the buds. Analysis of the expression of PpSUT2 during fruit development showed that the abundance of its transcripts increased at the end of April and then decreased to the lowest level at the end of July. Subcellular localization studies with the pCXDG vector as a probe demonstrated that PpSUT2 localized to cell membranes. An expression vector was constructed by inserting the PpSUT2 cDNA into pET32(a), and the vector was expressed in Escherichia coli (strain BL21) after induction with 1 mM isopropyl beta-D-1-thiogalactopyranoside at 25 degrees C. Analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified the induction of a 71-kDa protein. Further analysis indicated that PpSUT2 might be not directly involved in sucrose transport, instead, functioning as a sucrose sensor on the cytoplasmic membrane.

分类号：

  • 相关文献

[1]Molecular genetic characterization of rice seed lipoxygenase 3 and assessment of its effects on seed longevity. Long, Qizhang,Zhang, Wenwei,Wang, Peng,Shen, Wenbiao,Zhou, Tong,Liu, Nannan,Wang, Ren,Jiang, Ling,Huang, Jiexue,Wang, Yihua,Liu, Yuqiang,Wan, Jianmin,Wan, Jianmin.

[2]Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses. Wang, Lijuan,Ma, Fei,Shen, Wenbiao,Xu, Sheng,Wang, Ren,Zheng, Tianqing,Chen, Huiping,Chen, Huiping.

[3]Molecular cloning and expression analysis of the sucrose transporter gene family from Theobroma cacao L.. Lai, Jianxiong.

[4]Functional expression analysis of an acyl-ACP thioesterase FatB1 from Arachis hypogaea L. seeds in Escherichia coli. Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,He, Qingfang,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping. 2012

[5]Identification and expression analysis of three c-type lysozymes in Oreochromis aureus. Gao, Feng-ying,Qu, Lan,Ye, Xing,Tian, Yuan-yuan,Zhang, Li-li,Bai, Jun-jie,Lu, Maixin,Qu, Lan,Ye, Xing,Yu, Shao-guo. 2012

[6]The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses. Xiao-Xiao Cheng,Li, Zhi-Fang,Zhu, He-Qin,Li-Hong Zhao,Steven J. Klosterman,Hong-Jie Feng,Zi-Li Feng,Feng Wei,Yong-Qiang Shi,Zhi-Fang Li,He-Qin Zhu.

[7]Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon. Zhao, C.,Fu, M. J.,Qiu, L. H.,Zhao, C.,Fu, M. J.,Qiu, L. H.,Qiu, L. H.. 2016

[8]Characterization of three pheromone-binding proteins (PBPs) of Helicoverpa armigera (Hubner) and their binding properties. Mei, Xiang-Dong,Zhang, Yong-Jun,Guo, Yu-Yuan,Zhang, Tian-Tao,Feng, Ji-Nian,Guo, Yu-Yuan,Berg, Bente G.. 2012

[9]First Characterization of Sphingomyeline Phosphodiesterase Expression in the Bumblebee, Bombus lantschouensis. Han, L.,Dong, J.,Wang, Y.,Huang, J.,Wu, J.,Han, L.,He, S.. 2017

[10]Isolation and Expression Analysis of Catalase Genes in Erianthus arundinaceus and Sugarcane. Liu, Yang,Hu, Xiaowen,Yao, Yanli,Xu, Lei,Xing, Shulian. 2016

[11]Identification and expression analysis of the g-type and c-type lysozymes in grass carp Ctenopharyngodon idellus. Ye, Xing,Zhang, Lili,Tian, Yuanyuan,Tan, Aiping,Bai, Junjie,Li, Shengjie,Zhang, Lili.

[12]cDNA Cloning, Prokaryotic Expression, Polyclonal Antibody Preparation of the Auxin-Binding Protein 1 Gene from Grape Berry. Wan, Si-Bao,Wang, Wei,Luo, Mei,Huang, Wei-Dong,Zhan, Ji-Cheng,Wan, Si-Bao,Yin, Jing-Yuan,Wang, Wei.

[13]Cloning, silencing, and prokaryotic expression of strawberry sucrose synthase gene FaSus1. Hua, L. N.,Zang, M.,Wang, S. F.,Shen, Y. Y.,Guo, J. X.,Li, Y. Z..

[14]Detection of the Soybean Allergenic Protein Gly m Bd 28K by an Indirect Enzyme-Linked Immunosorbent Assay. Liu, Bin,Teng, Da,Wang, Xiumin,Wang, Jianhua,Liu, Bin,Teng, Da,Wang, Xiumin,Wang, Jianhua.

[15]Cloning and prokaryotic expression of Chinese sturgeons cystatin cDNA. Bai, J.,Ma, D.,Lao, H.,Jian, Q.,Ye, X.,Luo, J.. 2005

[16]Characterization and expression patterns of mannose-binding lectin (MMBL) gene in mulberry (Morus multicaulis) and its prokaryotic expression in E. coli. Li, R.,Chen, D.,Dominic, K.,Li, R.,Liu, L.,Zhao, W.,Li, R.,Wang, T.,Wang, Y.,Li, R.,Chen, D.,Dominic, K.,Li, R.,Liu, L.,Zhao, W.. 2017

[17]Cloning of a caffeoyl-coenzyme A O-methyltransferase from Camellia sinensis and analysis of its catalytic activity. Zhang, Yue,Lv, Hai-peng,Ma, Cheng-ying,Guo, Li,Tan, Jun-feng,Peng, Qun-hua,Lin, Zhi. 2015

[18]Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection. Ge, Junwei,Cui, Xingyang,Shi, Yunjia,Wei, Chengwei,Wen, Shanshan,Xia, Shuang,Zhao, Lili,Chen, Hongyan,Ge, Junwei. 2018

[19]Cloning and Functional Analysis of Thylakoidal Ascorbate Peroxidase (TAPX) Gene in Sugarcane. Zhang, Kun-Kun,Fan, Yan-Jiao,Yang, Li-Tao,Huang, Xing,Yang, Li-Tao,Li, Yang-Rui,Huang, Xing,Yang, Li-Tao,Li, Yang-Rui. 2015

[20]Recombinant expression, purification, and antimicrobial activity of a novel hybrid antimicrobial peptide LFT33. Feng, Xingjun,Guo, Jiayin,Song, Xueying,Li, Jing,Xu, Wenshan,Liu, Chunlong,Li, Zhongqiu. 2012

作者其他论文 更多>>

微信小程序