Undetectable off-target effects induced by FokI catalytic domain in mouse embryos

文献类型： 外文期刊

第一作者： Xie, Long

作者： Xie, Long;Feng, Hu;Li, Zhifang;Li, Di;Yang, Xiali;Yuan, Tanglong;Yan, Nana;He, Chenfei;Zheng, Jitan;Zuo, Zhenrui;Zheng, Yaxuan;Cao, Yaqi;Xiong, Xing Yao;Zuo, Erwei;Li, Di;Zheng, Jitan;Lu, Yangqing;Zheng, Yaxuan

作者机构：

期刊名称：GENOME BIOLOGY （ 影响因子：12.3； 五年影响因子：17.4 ）

ISSN： 1474-760X

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of off-target effects is essential for developing these tools. We use Genome-wide Off-target analysis by Two-cell embryo Injection (GOTI) to detect low-frequency off-target editing events in mouse embryos injected with FokI-based architectures. Specifically, we test FokI-heterodimers fused with TALENs, FokI homodimers fused with RYdCas9, or FokI catalytic domains alone resulting in no significant off-target effects. These FokI genome editing systems exhibit undetectable off-target effects in mouse embryos, supporting the further development of these systems for clinical applications.

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