A transcriptome-based screen of carboxylesterase-like genes that are involved in chlorpyrifos resistance in Laodelphax striatellus (Fallen)

文献类型： 外文期刊

第一作者： Zhang, Yueliang

作者： Zhang, Yueliang;Li, Guoqing;Fang, Jichao;Zhang, Yueliang;Wang, Lihua;Guo, Huifang;Zhang, Zhichun;Xie, Lin;Fang, Jichao

作者机构：

关键词： Laodelphax striatellus;Carboxylesterase;Chlorpyrifos;Metabolic resistance

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2012 年 104 卷 3 期

页码：

收录情况： SCI

摘要： Carboxylesterase (CarE)-mediated detoxification is one of the most important mediators of resistance to organophosphate insecticides (OPs). However, the molecular mechanisms by which CarEs mediate OP resistance are largely unknown. Our previous studies indicated that increased CarE activity was critical for chlorpyrifos (an OP) resistance in Laodelphax striatellus. In this study, 28 CarE and CarE-like genes (CarEs) were identified in the transcriptome of L. striatellus. Real-time quantitative PCR (qPCR) was performed to assess the relative expression of these 28 CarEs. Compared with the susceptible (YN) strain, Ls.CarE1 and Ls.CarE2 were significantly overexpressed in the chlorpyrifos-resistant (YN-CPF) strain by 32.06- and 8.52-fold, respectively. The expression levels of Ls.CarE1 and Ls.CarE2 were analyzed by qPCR to verify the correlation between overexpressed CarEs in the YN-CPF strain and chlorpyrifos resistance. Chlorpyrifos bioassays and CarE activity assays were subsequently performed on the chlorpyrifos relaxed selection (YN-RSF) strain. The results demonstrated that CarE activity was significantly higher in the YN-CPF and YN-RSF strains (4.15- and 1.92-fold, respectively) than in the YN strain, which was consistent with their respective degrees of chlorpyrifos resistance (214.0-fold for YN-CPF and 83.3-fold for YN-RSF). Ls.CarE1 was significantly overexpressed in both the YN-CPF and the YN-RSF strains (32.06-fold for YN-CPF and 8.6-fold for YN-RSF) compared with the YN strain, and a high degree of proportionality was observed between the expression level of Ls.CarE1 and the degree of resistance to chlorpyrifos across the three strains. However, the expression level of Ls.CarE2 in the YN-RSF and the YN strains was not significantly different. These data suggest that overexpressed Ls.CarE1 might be involved in chlorpyrifos resistance in the YN-CPF strain of L. striatellus. (c) 2012 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Cross-resistance and possible mechanisms of chlorpyrifos resistance in Laodelphax striatellus (Fallen). Wang, Lihua,Zhang, Yueliang,Liu, Yanhe,Fang, Jichao,Zhang, Yueliang,Han, Zhaojun,Liu, Yanhe. 2010

[2]Overexpression of carboxylesterase-1 and mutation (F439H) of acetylcholinesterase-1 are associated with chlorpyrifos resistance in Laodelphax striatellus. Zhang, Yueliang,Li, Shuo,Guo, Hui Fang,Zi, Jinyan,Wang, Lihua,Fang, Jichao,Xu, Lu,Zi, Jinyan,He, Peng. 2013

[3]Does Resistance to Buprofezin Improve Heat and Cold Tolerance of Laodelphax striatellus (Hemiptera: Delphacidae)?. Li, Yongteng,Liu, Xiangdong,Guo, Huifang,Zhang, Yueliang,Guo, Huifang.

[4]Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance. Yang, N.,Xie, W.,Jiao, X.,Yang, X.,Liu, B.,Li, R.,Zhang, Y.,Jones, C. M.,Bass, C.. 2013

[5]Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.). Deng, Wei,Li, Xuefeng,Zheng, Mingqi,Yu, Qin,Bai, Lianyang. 2016

[6]Overexpression of a P450 gene (CYP6CW1) in buprofezin-resistant Laodelphax striatellus (Fallen). Zhang, Yueliang,Guo, Huifang,Yang, Qiong,Li, Shuo,Wang, Lihua,Zhang, Gufeng,Fang, Jichao. 2012

[7]RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus. Fang, Ying,Lee, Seok Hee,Kim, Jong Hoon,Park, Dong Hwan,Park, Min Gu,Woo, Ra Mi,Lee, Bo Ram,Kim, Woo Jin,Je, Yeon Ho,Choi, Jae Young,Je, Yeon Ho,Li, Shuo. 2017

[8]Prediction of overseas migration of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae) in East Asia. Otuka, Akira,Matsumura, Masaya,Sanada-Morimura, Sachiyo,Zhou, Yijun,Lee, Gwan-Seok,Park, Hong-Hyun,Zhu, Yeqin,Liu, Zewen. 2012

[9]Lethal and sublethal effects of sulfoxaflor on the small brown planthopper Laodelphax striatellus. Xu, Lu,Gu, Zhong-Yan,Zhao, Chun-Qing,Zhang, Ya-Nan,Liu, Ying. 2016

[10]Presence of a short repeat sequence in internal transcribed spacer (ITS) 1 of the rRNA gene of Sogatella furcifera (Hemiptera: Delphacidae) from geographically different populations in Asia. Matsumoto, Yukiko,Sato, Yuki,Noda, Hiroaki,Fu, Qiang,Matsumura, Masaya.

[11]Identification and characterization of Laodelphax striatellus (Insecta: Hemiptera: Delphacidae) neutral sphingomyelinase. Zhou, Y.,Lin, X. -W.,Begum, M. -A.,Zhang, C. -H.,Shi, X. -X.,Jiao, W. -J.,Zhang, Y. -R.,Zhu, Z. -R.,Zhou, Y.,Lin, X. -W.,Begum, M. -A.,Zhang, C. -H.,Shi, X. -X.,Jiao, W. -J.,Zhang, Y. -R.,Zhu, Z. -R.,Zhou, Y.,Lin, X. -W.,Begum, M. -A.,Zhang, C. -H.,Shi, X. -X.,Jiao, W. -J.,Zhang, Y. -R.,Zhu, Z. -R.,Zhou, Y.,Yuan, J. -Q.,Li, H. -Y.,Yang, Q.,Mao, C..

[12]Resistance to cycloxaprid in Laodelphax striatellus is associated with altered expression of nicotinic acetylcholine receptor subunits. Zhang, Yueliang,Han, Yangchun,Yang, Qiong,Wang, Lihua,Guo, Huifang,Fang, Jichao,He, Peng,Liu, Zewen,Li, Zhong. 2018

[13]Bt rice expressing Cry2Aa does not cause direct detrimental effects on larvae of Chrysoperla sinica. Wang, Yuanyuan,Romeis, Joerg,Liu, Qingsong,Lin, Kejian,Chen, Xiuping,Peng, Yufa,Romeis, Joerg. 2013

[14]Studies on the epidemiology and yield losses from rice black-streaked dwarf disease in a recent epidemic in Zhejiang province, China. Wang, H. -D.,Jiang, X. -H.,Chen, J. -P.,Wang, A. -G.,Adams, M. J..

[15]Knockdown of NADPH-cytochrome P450 reductase results in reduced resistance to buprofezin in the small brown planthopper, Laodelphax striatellus (fallen). Zhang, Yueliang,Wang, Yaming,Wang, Lihua,Yao, Jing,Guo, Huifang,Fang, Jichao,Wang, Yaming.

[16]Resistance monitoring and assessment of resistance risk to pymetrozine in laodelphax striatellus (Hemiptera: Delphacidae). Ban, Lanfeng,Huang, Ziyang,Peng, Yongqiang,Gao, Congfen,Zhang, Shuai,He, Yueping.

[17]Influence of Pymetrozine on Feeding Behaviors of Three Rice Planthoppers and a Rice Leafhopper using Electrical Penetration Graphs. He, Yueping,Zhang, Juefeng,Chen, Jianming,Chen, Li,Chen, Liezhong,Xiao, Pengfei,Wu, Quancong,Zhu, Yu Cheng.

[18]ARGONAUTE SUBFAMILY GENES IN THE SMALL BROWN PLANTHOPPER, Laodelphax striatellus (HEMIPTERA: DELPHACIDAE). Zhou, Yan-Ru,Zhou, Yan-Ru,Li, Jun-Min,Sun, Zong-Tao,Xie, Li,Chen, Jian-Ping,Li, Lin-Ying.

[19]The cross-resistance patterns and biochemical characteristics of an imidadoprid-resistant strain of the cotton aphid. Chen, Xiaokun,Wang, Jie,Wang, Kaiyun,Xia, Xiaoming,Shi, Xugen,Wang, Hongyan.

[20]Effect of Dietary Cadmium on the Activity of Glutathione S-Transferase and Carboxylesterase in Different Developmental Stages of the Oxya chinensis (Orthoptera: Acridoidea). Zhang, Y. P.,Song, D. N.,Wu, H. H.,Yang, H. M.,Zhang, J. Z.,Ma, E. B.,Guo, Y. P.,Zhang, Y. P.,Li, L. J.. 2014

作者其他论文 更多>>

微信小程序