Antiviral activity of rChIFN-alpha against vesicular stomatitis virus and Newcastle disease virus: A novel recombinant chicken interferon-alpha showed high antiviral activity
文献类型: 外文期刊
第一作者: Hou, Fengxiang
作者: Hou, Fengxiang;Liu, Ke;Shen, Ting;Zhou, Bin;Cao, Ruibin;Li, Peide;Chen, Puyan;Hou, Fengxiang
作者机构:
关键词: Chicken interferon;Newcastle disease virus;Vesicular stomatitis virus
期刊名称:RESEARCH IN VETERINARY SCIENCE ( 影响因子:2.534; 五年影响因子:2.382 )
ISSN: 0034-5288
年卷期: 2011 年 91 卷 3 期
页码:
收录情况: SCI
摘要: The sequences of 27 chicken interferon-alpha (ChIFN-alpha) genes were obtained from GenBank. The gene sequences were compared and homology between them was determined by using a bio-software. On the basis of these results, a new rChIEN-alpha peptide sequence with 194 amino acids was assembled. Thereafter, on the basis of the new amino acid sequences and by using the most frequently occurring codes of Pichia pastoris, and a 582 bp gene sequence was formed. In order to amplify this non-templated gene, 16 primers were designed, and their gene sequences were synthesized, and amplified. This amplified gene sequence was cloned into the expression vector pPICZ alpha-A to construct a recombination plasmid named pPICZ-rChIFN-alpha. Then, the recombination plasmid was induced to express the rChIFN-alpha protein. The results demonstrated that the recombinant plasmid pPICZ-rChIFN-alpha was successfully expressed in P. pastoris. Furthermore, rChIFN-alpha had a considerable antiviral activity against both Newcastle disease virus (NDV) and vesicular stomatitis virus (VSV). Therefore, this method of gene engineering could give direction to research on the key amino acids in the interferon or analogous proteins and enable the construction of proteins with high antiviral activity, which can be used both for research and industrial purposes. (C) 2010 Elsevier Ltd. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Classical swine fever virus recruits ALIX and ESCRT-III to facilitate viral budding
作者:Chen, Jinxia;Yang, Hanfei;Wan, Mingyue;Cheng, Yan;Bai, Jishan;Li, Yuhang;Chen, Jing;Zhao, Bingqian;Zhou, Bin;Gao, Fei
关键词:CSFV; budding; ESCRT; ALIX; vesicle transport
-
Gallic acid enhances honeybee larvae resistance to Paenibacillus larvae infections: Insights from molecular docking, bacterial community modulation, and whole-genome sequencing
作者:Ye, Man-Hong;Jiang, Yin-Hong;Li, Xiao-Yuan;Han, Qian-Nan;Meng, Chuang;Ye, Man-Hong;Meng, Chuang;Ji, Feng;Zhou, Bin
关键词:American foulbrood disease; gallic acid; honeybee larvae; microbiota modulation; molecular docking; Paenibacillus larvae
-
Probiotic Potential of Enterococcus lactis GL3 Strain Isolated from Honeybee (Apis mellifera L.) Larvae: Insights into Its Antimicrobial Activity Against Paenibacillus larvae
作者:Ye, Manhong;Jiang, Yinhong;Han, Qiannan;Li, Xiaoyuan;Ye, Manhong;Meng, Chuang;Ji, Chao;Ji, Feng;Zhou, Bin
关键词:honeybee larvae; lactic acid bacteria;
Paenibacillus larvae ;Enterococcus lactis ; aborycin -
Development of a Noninfectious Japanese Encephalitis Virus Replicon for Antiviral Drug Screening and Gene Function Studies
作者:Yang, Yang;Zheng, Jiayang;Lin, Yafang;Zhang, Hailong;Zhang, Junjie;Li, Zongjie;Liu, Ke;Li, Beibei;Shao, Donghua;Qiu, Yafeng;Ma, Zhiyong;Wei, Jianchao;Zhang, Yan;Zhao, Qianming
关键词:Japanese encephalitis virus; replicon; green fluorescent protein; antiviral drug screening; RNA replication
-
Sheep serve as amplifying hosts of Japanese encephalitis virus, increasing the risk of human infection
作者:Zhang, Hailong;Li, Dan;Zheng, Jiayang;Qiu, Yafeng;Liu, Ke;Li, Zongjie;Li, Beibei;Shao, Donghua;Ma, Zhiyong;Wei, Jianchao;Zhang, Hailong;Liu, Juxiang;Bao, Jingyue;Wang, Zhiliang
关键词:
-
Unlocking phosphorus resources: phosphate-solubilizing microorganisms as a green strategy for activating phosphorus in acidic red soils and promoting crop growth
作者:Ma, Qingqing;Yang, Yuting;Zhou, Bin;Ma, Qingqing;Chen, Huayi;Yang, Yuting;Zhou, Bin
关键词:microbial technology; red soil improvement; phosphorus inactivation; agricultural sustainability; tropical and subtropical agriculture
-
Partial protective efficacy of the current licensed Japanese encephalitis live vaccine against the emerging genotype I Japanese encephalitis virus isolated from sheep
作者:Zhang, Hailong;Liu, Juxiang;Zhang, Yan;Li, Dan;Zheng, Jiayang;Zhang, Junjie;Li, Zongjie;Liu, Ke;Li, Beibei;Shao, Donghua;Qiu, Yafeng;Ma, Zhiyong;Wei, Jianchao
关键词:Japanese encephalitis virus; sheep; SA14-14-2 vaccine; neutralizing antibodies; protective efficacy