Diversity of the Predominant Spoilage Bacteria in Water-Boiled Salted Duck during Storage

文献类型: 外文期刊

第一作者: Liu, Fang

作者: Liu, Fang;Wang, Daoying;Zhu, Yongzhi;Xu, Weimin;Du, Lihui

作者机构:

关键词: denaturing gradient gel electrophoresis;preservation;16S ribosome ribonucleic acid;spoilage bacteria;water-boiled salted duck

期刊名称:JOURNAL OF FOOD SCIENCE ( 影响因子:3.167; 五年影响因子:3.376 )

ISSN: 0022-1147

年卷期: 2010 年 75 卷 5 期

页码:

收录情况: SCI

摘要: The spoilage microbiota in water-boiled salted duck during storage at 4 degrees C was determined using culture-dependent and independent methods. Analysis of the denaturing gradient gel electrophoresis (DGGE) patterns of PCR amplicons targeting the V3 region of the 16S rDNA and sequencing of the bands allowed profiling of the microbiota present in the duck. Community DNA extracts were prepared directly from water-boiled salted duck and from culturable bacterial fractions harvested from both MRS and PCA media. The spoilage bacteria mainly consisted of Staphylococcus saprophyticus, Macrococcus caseolyticus, Weissella, Halomonas sp. or Cobetia sp., and Exiguobacterium sp. based on sequencing and homology search of the DGGE bands. It appeared that both the bacterial counts and diversity increased during storage time. By plating method, bacterial counts in MRS agar increased from 10(4) to 10(8) CFU/g from day 1 to 10, while total bacterial counts in PCA agar reached 10(9) CFU/g after 10 d. Total of 14 strains isolated from PCA and MRS agar were identified as M. caseolyticus (2), S. saprophyticus (7), S. sciuri (1), W. paramesenteroides (2), and W. confusa (2) by 16S rDNA sequencing. The identification of the spoilage-related microbiota is helpful to better understand the bacteria ecology in water-boiled salted duck and may lead to the discovery of appropriate preservation strategies.

分类号:

  • 相关文献

[1]Inhibitory effects of chitosan combined with nisin on Shewanella spp. isolated from Pseudosciaena crocea. He, Mu,Guo, Quan-you,Song, Wei,Zhang, Guo-wen,Li, Bao-guo.

[2]Effect of Poly-epsilon-lysine on vacuum packed tilapia stored at 4 degrees C. Ma, Haixia,Cheng, Linli,Li, Laihao,Yang, Xianqing. 2014

[3]Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.). Wang Xin-min,Yin Gui-xiang,Wang Ke,Du Li-pu,Xu Hui-jun,Ye Xing-guo,Ren Xian,Li Jia-rui. 2014

[4]Chestnut Genebank in China National Clonal Plant Germplasm Repository. Li Guo-tian,Ai Cheng-xiang,Zhang Li-si,Wei Hai-rong,Liu Qing-zhong. 2009

[5]Tea polyphenols incorporated into alginate-based edible coating for quality maintenance of Chinese winter jujube under ambient temperature. Zhang, Lihua,Dong, Yu,Zhi, Huanhuan,Zong, Wei,Zhang, Lihua,Dong, Yu,Zhi, Huanhuan,Zong, Wei,Li, Shunfeng. 2016

[6]The effect of an inoculant and enzymes on fermentation and nutritive value of sorghum straw silages. Xing, L.,Chen, L. J.,Han, L. J.,Xing, L..

[7]The host species affects the microbial community in the goat rumen. Shi, P. J.,Meng, K.,Zhou, Z. G.,Wang, Y. R.,Diao, Q. Y.,Yao, B.. 2008

[8]Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development. Wei, M.,Tan, F.,Zhu, H.,Cheng, K.,Wu, X.,Wang, J.,Zhao, K.,Tang, X.,Wei, M.,Tan, F.,Zhu, H.,Cheng, K.,Wu, X.,Wang, J.,Zhao, K.,Tang, X..

[9]Effect of Bacillus cereus as a water or feed additive on the gut microbiota and immunological parameters of Nile tilapia. Wang, Miao,Lu, Maixin,Ke, Xiaoli,Liu, Zhigang,Gao, Fengying,Cao, Jianmeng,Zhu, Huaping,Yi, Mengmeng,Yu, Deguang,Liu, Guanbin.

[10]The application of bioflocs technology in high-intensive, zero exchange farming systems of Marsupenaeus japonicus. Zhao, Pei,Huang, Jie,Wang, Xiu-Hua,Song, Xiao-Ling,Yang, Cong-Hai,Zhang, Xu-Guang,Wang, Guo-Cheng,Zhao, Pei,Zhang, Xu-Guang,Wang, Guo-Cheng. 2012

[11]The influence of Bt-transgenic maize pollen on the bacterial diversity in the midgut of Apis mellifera ligustica. Cui, Hong-Juan,Shu, Chang-Long,Song, Fu-Ping,Zhang, Jie,Dai, Ping-Li,Zhou, Ting,Lang, Zhi-Hong. 2013

[12]Effects of inclusion of waste vinegar residue in the diet of laying hens on chyme characteristics and gut microflora. Song, Z. T.,Dong, X. F.,Tong, J. M.,Wang, Z. H..

[13]Bacterial Community of Koumiss from Mongolia Investigated by Culture and Culture-Independent Methods. Ringo, Einar,Andersen, Renate,Breines, Eva Marie,Hareide, Ellinor,Yttergard, Gerd Jorun,Opsal, Kirstin,Johansen, Heidi Mathilde,Andreassen, Ann Kristin,Kousha, Armin,Godfroid, Jacques,Sperstad, Sigmund,Zhou, Zhigang,Ren, Pen,Holzapfel, Wilhelm.

[14]Molecular analysis of intestinal bacterial microbiota of broiler chickens fed diets containing fermented cottonseed meal. Sun, H.,Fang, C. L.,Feng, J.,Tang, J. W.,Yao, X. H.,Wu, Y. F.,Wang, X.. 2013

[15]Identification of lactic acid bacteria in the feces of dairy cows fed whole crop maize silage to assess the survival of silage bacteria in the gut. Han, Hongyan,Li, Guangpeng,Wang, Chao,Li, Yanbing,Yu, Zhu,Xu, Qingfang,Tang Thuy Minh,Nishino, Naoki. 2018

[16]Bacterial community dynamics in a biodenitrification reactor packed with polylactic acid/poly (3-hydroxybutyrate-co-3-hydroxyvalerate) blend as the carbon source and biofilm carrier. Qiu, Tianlei,Xu, Ying,Gao, Min,Han, Meilin,Wang, Xuming,Xu, Ying,Wang, Xuming.

[17]Effects of alfalfa meal on the intestinal microbial diversity and immunity of growing ducks. Jiang, J. F.,Song, X. M.,Wu, J. L.,Jiang, Y. Q..

[18]The effect of cultivar, wilting and storage period on fermentation and the clostridial community of alfalfa silage. Zheng, Mingli,Niu, Dongze,Zuo, Sasa,Xu, Chuncheng,Mao, Peichun,Meng, Lin. 2018

[19]Effects of dietary 60 g kg(-1) dried distiller's grains in least-cost practical diets on production and gut allochthonous bacterial composition of cage-cultured fish: comparison among fish species with different natural food habits. He, S.,Liu, Y.,Xu, L.,Zhou, Z.,Yao, B.,Wu, Z.,Wu, N.,Tao, Y.,Ringo, E..

[20]Metabolic and phylogenetic profiles of microbial communities from a mariculture base on the Chinese Guangdong coast. Hu, Xiaojuan,Wen, Guoliang,Cao, Yucheng,Li, Zhuojia,Hu, Xiaojuan,Yang, Yufeng,Hu, Xiaojuan,Gong, Yingxue,He, Zhili,He, Zhili.

作者其他论文 更多>>