Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae)
文献类型: 外文期刊
第一作者: Jiang, Xiaojing
作者: Jiang, Xiaojing;Jiang, Weihua;Han, Zhaojun;Qu, Mingjing;Denholm, Ian;Fang, Jichao
作者机构:
关键词: Chilo suppressalis;Acetylcholinesterase;Gene mutation;Insecticide resistance;Triazophos
期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )
ISSN: 0006-291X
年卷期: 2009 年 378 卷 2 期
页码:
收录情况: SCI
摘要: Two full-length genes encoding different acetylcholinesterases (AChEs), designated as Ch-ace1 and Ch-ace2 were cloned from strains of the rice stern borer (Chilo suppressalis) susceptible and resistant to the organophosphate insecticide triazophos. Sequence analysis found an amino acid Mutation A314S in Ch-ace1 (corresponding to A201 in Torpedo californica AChE) that was consistently associated with the occurrence of resistance. This mutation removed an MspA1 I restriction site from the wild type allele. An assay based on restriction fragment length polymorphism (RFLP) analysis was developed to diagnose A314S genotypes in held populations. Results showed a strong correlation between frequencies of the Mutation and phenotypic levels of resistance to triazophos. The assay offers a prospect for rapid monitoring of resistance and assisting with the appropriate choice of insecticide for combating damage caused by C. suppressalis. 2008 Elsevier Inc. All rights reserved.
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