Compound Chinese herbal medicinal ingredients can enhance immune response and efficacy of RHD vaccine in rabbit
文献类型: 外文期刊
第一作者: Yang, Longsheng
作者: Yang, Longsheng;Hu, Yuanliang;Wang, Deyun;Kong, Xiangfeng;Yang, Longsheng;Xue, Jiabin;Wang, Fang;Xu, Weizhong;Li, Peng;Kong, Xiangfeng;Kong, Xiangfeng
作者机构:
关键词: compound Chinese herbal medicinal ingredients;rabbit hemorrhagic disease;lymphocyte proliferation;gene expression;antibody titer
期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )
ISSN: 0264-410X
年卷期: 2008 年 26 卷 35 期
页码:
收录情况: SCI
摘要: In order to validate the immune-enhancement efficacy of compound Chinese herbal medicinal ingredients (cCHMIs), made with astragalus polysaccharide (APS), epimedium polysaccharide (EPS), propolis flavone (PF) and ginsenosides (GS), as immune potentiator or vaccine adjuvants for rabbits, the effects of two cCHMIs on rabbit lymphocyte proliferation and IFN-gamma and IL-10 mRNA expression of T lymphocyte in vitro were determined. At the same time, two cCHMIs were injected into 35-day-old rabbits after mixed with rabbit hemorrhagic disease (RHD) vaccine taking aluminum adjuvant and phosphate-buffered saline (PBS) as controls. On days 7, 14, 21,35 and 49 after the vaccination, the dynamic changes of peripheral lymphocyte proliferation and serum antibody titers of the rabbits were analyzed. On day 63, all rabbits were challenged with RHD virus. The results showed that the two cCHMIs could significantly promote rabbit lymphocyte proliferation and IFN-gamma and IL-10 mRNA expression of T lymphocyte in vitro. In vivo, two cCHMIs could significantly enhance serum antibody titers and lymphocyte proliferation. Their adjuvanticity was slightly superior to aluminum adjuvant. All the rabbits vaccinated with the cCHMIs adjuvant vaccine were protected. These findings confirmed that two cCHMIs possessed better immune-enhancement efficacy and would be used as effective immune adjuvant of RHD vaccine. (C) 2008 Elsevier Ltd. All rights reserved.
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