Complete nucleotide sequence of a novel porcine circo-virus-like agent and its infectivity in vitro

文献类型： 外文期刊

第一作者： Wen LiBin

作者： Wen LiBin;He KongWang;Ni YanXiu;Zhang XueHan;Guo RongLi;Pan QunXin;Yang HanChun

作者机构：

关键词： P2;porcine circovirus;molecular clone;PK-15 cells

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN： 1006-9305

年卷期： 2008 年 51 卷 5 期

页码：

收录情况： SCI

摘要： A novel agent (hence termed as P2) was isolated from pig sera in China, which contained covalently bound circular genomic DNAs of 993 nucleotides. Sequence analyses indicated that the agent was closely related to the porcine circovirus (PCV). The molecular clone of P2 was constructed subsequently and used for the following studies. Intracytoplasmic inclusions and intranuclear inclusions were only found in PK-15 cells transfected with the tandem dimer of P2 molecular DNA clone. Intracytoplasmic inclusions were round or irregular in shape and 0.1-0.4 mu m in diameter, and intranuclear inclusions were electronically denser than intracytoplasmic inclusions and had two general shapes: round/small (0.1 mu m in diameter) and hexagonal/large (0.5-1.4 mu m in diameter). The inclusions were not membranously bound. The cells transfected with the tandem dimer of P2 molecular DNA clone were tested positive for P2 DNA at passages 5. The P2 antigen could be detected in both transfected and passaged PK-15 cells. This is the first report regarding the complete nucleotide sequence of a small DNA genome in a circovirus-like infectious agent in vitro.

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