Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C(4) photosynthesis enzymes

文献类型: 外文期刊

第一作者: Jiao, DM

作者: Jiao, DM;Huang, XQ;Li, X;Chi, W;Kuang, TY;Zhang, QD;Ku, MSB;Cho, DH

作者机构:

关键词: C4 photosynthesis enzymes;carbon assimilation;photosynthetic characteristics;photo-oxidation;transgenic rice

期刊名称:PHOTOSYNTHESIS RESEARCH ( 影响因子:3.573; 五年影响因子:3.674 )

ISSN: 0166-8595

年卷期: 2002 年 72 卷 1 期

页码:

收录情况: SCI

摘要: The photosynthetic characteristics of four transgenic rice lines over-expressing rice NADP-malic enzyme (ME), and maize phosphoenolpyruvate carboxylase (PC), pyruvate,orthophosphate dikinase (PK), and PC+PK (CK) were investigated using outdoor-grown plants. Relative to untransformed wild-type (WT) rice, PC transgenic rice exhibited high PC activity (25-fold increase) and enhanced activity of carbonic anhydrase (more than two-fold increase), while the activity of ribulose-bisphosphate carboxylase/oxygenase (Rubisco) and its kinetic property were not significantly altered. The PC transgenic plants also showed a higher light intensity for saturation of photosynthesis, higher photosynthetic CO(2) uptake rate and carboxylation efficiency, and slightly reduced CO(2) compensation point. In addition, chlorophyll a fluorescence analysis indicates that PC transgenic plants are more tolerant to photo-oxidative stress, due to a higher capacity to quench excess light energy via photochemical and non-photochemical means. Furthermore, PC and CK transgenic rice produced 22-24% more grains than WT plants. Taken together, these results suggest that expression of maize C(4) photosynthesis enzymes in rice, a C(3) plant, can improve its photosynthetic capacity with enhanced tolerance to photo-oxidation.

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