SSAP analysis reveals candidate genes associated with deastringency in persimmon (Diospyros kaki Thunb.) treated with 40 degrees C water

文献类型： 外文期刊

第一作者： Guan, Changfei

作者： Guan, Changfei;Chen, Li;Chen, Wenxing;Mo, Rongli;Zhang, Qinglin;Liu, Jihong;Luo, Zhengrong;Du, Xiaoyun

作者机构：

关键词： Persimmon;40 degrees C water;Deastringency;SSAP;Transcript-derived fragments;qRT-PCR

期刊名称：TREE GENETICS & GENOMES （ 影响因子：2.297； 五年影响因子：2.547 ）

ISSN： 1614-2942

年卷期： 2015 年 11 卷 2 期

页码：

收录情况： SCI

摘要： Warm water treatment is a simple and effective deastringency method for the persimmon fruit. The astringency in the 'Eshi 1' persimmon, a Chinese pollination-constant and non-astringent (C-PCNA) cultivar, can be quickly removed in 40 degrees C water coupled with the activation of the retrotransposon DkRE1. In this study, transcriptional level changes in 'Eshi 1' persimmon treated with 40 degrees C water were studied with a sequence-specific amplification polymorphism (SSAP) approach to investigate the genes associated with deastringency. The complementary DNA (cDNA)-SSAP analysis revealed that 283 differentially expressed transcript-derived fragments (TDFs) potentially involved in 'Eshi 1' persimmon deastringency were successfully cloned and sequenced. In total, 116 differentially expressed TDFs were annotated as genes with known function, including four aldehyde metabolism genes and two pectin-related genes. Our data suggested that the tannin-pectin complex formation and the aldehyde-mediated coagulation effect contributed to the deastringency of the persimmon fruit treated with 40 degrees C water. Notably, three gene fragments, triosephosphate isomerase (TDF 175-3), pyruvate kinase (TDF 284-2), and neomenthol dehydrogenase (TDF 238-2), which are potentially involved in the 40 degrees C water coagulation effect were identified. To our knowledge, this report is the first to identify these gene fragments in the persimmon. The SSAP method was innovatively applied to gene discovery based on high-density polymorphisms. Expression analysis and transcriptome data confirmed the validity and accuracy of the cDNA-SSAP method for discovering new candidate genes. These successfully isolated genes could facilitate understanding of astringency removal in persimmon.

分类号：

  • 相关文献

[1]Isolation and Characterization of DkPK Genes Associated with Natural Deastringency in C-PCNA Persimmon. Guan, Changfei,Chen, Wenxing,Mo, Rongli,Zhang, Qinglin,Luo, Zhengrong,Du, Xiaoyun,Zhang, Qinglin,Luo, Zhengrong. 2016

[2]Development of Ty1-copia retrotransposon-based SSAP molecular markers for the study of genetic diversity in peach. Jiao, Yun,Ma, Rui-juan,Shen, Zhi-jun,Yu, Ming-liang.

[3]Comparative expression analysis in susceptible and resistant Gossypium hirsutum responding to Verticillium dahliae infection by cDNA-AFLP. Wang, Sheng-Zheng,Liu, Kai,Si, Ning,Qi, Fang-Jun,Jian, Gui-Liang.

[4]cDNA-AFLP analysis reveals heat shock proteins play important roles in mediating cold, heat, and drought tolerance in Ammopiptanthus mongolicus. Guo, Huiming,Li, Zhaochun,Zhou, Meiliang,Cheng, Hongmei.

[5]Transferability of retrotransposon primers derived from Persimmon (Diospyros kaki Thunb.) across other plant species. Du, X. Y.,Wang, Y. B.,Du, X. Y.,Hu, Q. N.,Zhang, Q. L.,Luo, Z. R.. 2013

[6]Genome-wide transcriptome analysis of Chinese pollination-constant nonastringent persimmon fruit treated with ethanol. Luo, Chun,Zhang, Qinglin,Luo, Zhengrong,Luo, Chun. 2014

[7]Evolution of the antioxidant capacity and phenolic contents of persimmon during fermentation. Zou, Bo,Wu, Jijun,Yu, Yuanshan,Xiao, Gengsheng,Xu, Yujuan. 2017

[8]Identification of xyloglucan endotransglucosylase/hydrolase genes (XTHs) and their expression in persimmon fruit as influenced by 1-methylcyclopropene and gibberellic acid during storage at ambient temperature. Zhu, Qinggang,Zhang, Zhengke,Rao, Jingping,Lv, Jingyi,Hou, Yali,Song, Kanghua,Zhang, Zhengke,Huber, Donald J.. 2013

[9]Expression of Expansin Gene (CDK-Exp3) and Its Modulation by Exogenous Gibberellic Acid During Ripening and Softening of Persimmon Fruit. Zhang, Zhengke,Fu, Runshan,Rao, Jingping,Chang, Xiaoxiao,Zhang, Yu,Jiang, Nina,Zhang, Zhengke,Hu, Meijiao,Huber, Donald J.. 2012

[10]Genetic Diversity of Persimmon Landraces as Assessed by Morphological Traits and IRAP Molecular Markers in Jiangxi Province. Du, X.,Zen, M.,Wang, Y.,Xiong, Y.,Huang, J.,Le, M.,Pei, X.,Luo, Z..

[11]Molecular Cloning, Structural Characterization, and Ripening-Related Expression of Lipoxygenase Genes from Three Persimmon Cultivars Differing in Postharvest Ripening Rate. Hou, Yali,Ban, Qiuyan,Zhang, Ting,Rao, Jingping,Huber, Donald J.,Dong, Xiaoqing,Chang, Xiaoxiao,Zhang, Ting.

[12]GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L). Fengli Zhao,JianhuiMa,Libei Li,Shuli Fan,Yaning Guo,Meizhen Song,Hengling Wei,Chaoyou Pang,Shuxun Yu. 2016

[13]A Novel AP2/ERF Transcription Factor CR1 Regulates the Accumulation of Vindoline and Serpentine in Catharanthus roseus. Gao, Fangyuan,Ren, Juansheng,Lu, Xianjun,Ren, Guangjun,Wang, Rui. 2017

[14]Molecular characterization and expression patterns of PsSVP genes reveal distinct roles in flower bud abortion and flowering in tree peony (Paeonia suffruticosa). Wang, Shunli,Xue, Jingqi,Zhu, Fuyong,Ren, Xiuxia,Zhang, Xiuxin,Wang, Shunli,Xue, Jingqi,Zhu, Fuyong,Ren, Xiuxia,Zhang, Xiuxin,Ahmadi, Noorollah,Holloway, Patricia. 2014

[15]Molecular Cloning and Induced Expression of Six Small Heat Shock Proteins Mediating Cold-Hardiness in Harmonia axyridis (Coleoptera: Coccinellidae). Wang, Hui-Juan,Shi, Zuo-Kun,Shen, Qi-Da,Xu, Cai-Di,Wang, Shi-Gui,Tang, Bin,Wang, Bing,Wang, Su,Meng, Zhao-Jun. 2017

[16]Cloning, characterization, and expression of a novel member of proteasomal subunits gene in turbot, Scophthalmus maximus. Zhang Bo,Song Wenping,Zheng Debin,Ma Chao,Xiao Guangxia,Wang Xianli. 2015

[17]Characterization of target mRNAs for Prunus persica microRNAs using an integrated strategy of PLM-RACE, PPM-RACE and qRT-PCR. Zhang, Yanping,Han, Jian,Pervaiz, Tariq,Fang, Jinggui,Yu, Mingliang,Ma, Ruijuan. 2014

[18]Transcript Profiling of Hevea brasiliensis during Latex Flow. Tian, Wei-Min. 2017

[19]Selection of reliable reference genes for quantitative real-time PCR gene expression analysis in Jute (Corchorus capsularis) under stress treatments. Niu, Xiaoping,Qi, Jianmin,Zhang, Gaoyang,Xu, Jiantang,Tao, Aifen,Fang, Pingping,Zhang, Gaoyang,Su, Jianguang. 2015

[20]Cloning and expression analysis of two different LhcSR genes involved in stress adaptation in an Antarctic microalga, Chlamydomonas sp ICE-L. Mou, Shanli,Liang, Qiang,Miao, Jinlai,Zheng, Zhou,Zhang, Xiaowen,Ye, Naihao,Xu, Dong,Dong, Meitao,Liang, Chengwei. 2012

作者其他论文 更多>>

微信小程序