Extraction and Detection of Total RNA from Flammulina Velutipes

文献类型： 外文期刊

第一作者： Jiang, Bo-Quan

作者： Jiang, Bo-Quan;Li, Min;Chen, Liu-Meng

作者机构：

关键词： Space Flammulina Velutipes;RNA;Extraction;Quanidine Thiocyanate;STE;Trizol

期刊名称：2015 INTERNATIONAL CONFERENCE ON ENVIRONMENT, MANUFACTURING INDUSTRY AND ECONOMIC DEVELOPMENT, (EMIED 2015)

ISSN：

年卷期： 2015 年

页码：

收录情况： SCI

摘要： To extract total RNA from biomass is an important experimental technology frequently used in modern molecular biology research. The three method of guanidine thiocyanate, STE and Trizol reagent were used to extract total RNA from flammulina velutipes. The operation processes of the three methods were described in detail. Formaldehyde agarose gel electrophoresis was used to detect the total RNA. The results show that all the three methods are suitable for the molecular biology research related to space flammulina velutipes, and guanidine thiocyanate method is the best one for the genome RNA extraction from space flammulina velutipes. With suitable precipitation time and sample weight (5 ul), more and purer total RNA were extracted and the ratio of 28s/18s electrophoresis brightness was about 2: 1. The extraction of total RNA is of great significance for the subsequent molecular experimental study on RT-pcr, gene library construction, etc.

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