Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat

文献类型： 外文期刊

第一作者： Meng Ya-ning

作者： Meng Ya-ning;Kang Su-hua;Lan Su-que;Li Xing-pu;Zhang Ye-lun;Bai Feng

作者机构：

关键词： chromosome;dwarfing gene;genetic distance;linkage analysis;molecular markers;polymorphism;Triticum aestivum L.

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2013 年 12 卷 5 期

页码：

收录情况： SCI

摘要： A segregating population with 410 F-2 individuals from the cross MERCIA (Rht-B1a) x Dwarf 123 was made to identify a new major dwarfing gene carrying by novel wheat germplasm Dwarf 123. Combination of bulk segerant analysis method was used. A total of 145 SSR markers were tested for polymorphisms among parental lines and DNA bulks of F-2 population. Out of 145 primer pairs only three markers revealed corresponding polymorphism among parental lines and F-2 DNA bulks. The marker Barc20 was close to the dwarfing gene with a genetic distance of 1.8 cM, and markers Gwm513 and Gwm495 were linked to the gene with genetic distance of 6.7 and 13 cM, respectively. Linkage analysis mapped the dwarfing gene to the long arm of chromosome 4B with the order of Barc20-dwarfing gene-Gwm513-Gwm495. The Comparision between the new gene and the known Rht-B1 alleles showed that dwarfing gene Rht-Ai123 was different from the others. The identification of the new dwarfing gene and its linked markers will greatly facilitate its utilization in wheat high yield breeding for reducing plant height.

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