A SERS-based multiple immuno-nanoprobe for ultrasensitive detection of neomycin and quinolone antibiotics via a lateral flow assay

文献类型： 外文期刊

第一作者： Shi, Qiaoqiao

作者： Shi, Qiaoqiao;Zhang, Gaiping;Shi, Qiaoqiao;Sun, Yaning;Deng, Ruiguang;Teng, Man;Li, Qingmei;Yang, Yanyan;Hu, Xiaofei;Zhang, Gaiping;Shi, Qiaoqiao;Huang, Jie;Zhang, Zhijun;Zhang, Gaiping

作者机构：

关键词： Gold nanoparticles;4-aminothiophenol;Monoclonal antibody;Broad-specificity;Optimization;Spectra;Standard curve;Cross-reactivity;Affix recovery;Milk analysis

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN： 0026-3672

年卷期： 2018 年 185 卷 2 期

页码：

收录情况： SCI

摘要： The authors describe an ultrasensitive method for simultaneous detection of neomycin (NEO) and quinolones antibiotics (QNS). It is based on the use of (a) two immuno-nanoprobes (a probe for NEO and a probe for QNS), (b) surface-enhanced Raman scattering (SERS) detection, and (c), a portable lateral flow assay (LFA). The two probes consist of gold nanoparticles (AuNPs) conjugated to the Raman active molecule 4-aminothiophenol (PATP), and to monoclonal antibody against NEO (NEO mAb) or against NOR (NOR mAb). Quantitative detection of NEO and QNS was realized via SERS of the PATP-coated AuNPs captured in the test line of a LFA. Under optimized condition, the visual limits of LFA are 10 ng.mL(-1) for NEO and 200 ng.mL(-1) for NOR, and with LODs down to 0.37 pg.mL(-1) and 0.55 pg.mL(-1) by using SERS. The NEO test line is not interfered by the NEO analogues gentamycin, streptomycin and tobramycin, but the NOR test line suffers from different degrees of cross-reactivity (CR) to 12 common other QNS, the CRs ranging from 1.5% to 136%. The recoveries of NEO and NOR from spiked milk samples ranged between 86% and 121%, with relative standard deviations (RSD) from 3% to 6%. The method is highly sensitive, accurate and effective. It may be applied to simultaneous detection of NEO and 8 QNS, including NOR, enoxacin, ciprofloxacin, ofloxacin, fleroxacin, marbofloxacin, enrofloxacin, and pefloxacin.

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