Anti-inflammatory activity of cecropin-A2 from Musca domestica

文献类型： 外文期刊

第一作者： Wei, Rui-Yang

作者： Wei, Rui-Yang;Zhao, Meng-Fei;Wei, Rui-Yang;Bai, Jie;Zhao, Meng-Fei;Xu, Bin;Li, Wen-Jia;Wei, Feng-Xian;Xi, Yan-Yan;Li, Shao-Yu

作者机构：

关键词： Cecropin;S. aureus;LTA;Proinflammatory cytokines;NF kappa B;Transcript variant

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2017 年 110 卷

页码：

收录情况： SCI

摘要： This study aimed to investigate the anti-inflammatory activity of Musca domestica cecropin-A2 (Mdc-A2) toward Staphylococcus aureus (S. aureus) to learn more about their immunological functions. RAW264.7 cells were transfected with recombinant lentiviruses introduce pLEX-Mdc-A2into the RAW264.7 cell line (RAW-Mdc-A2). The RAW264.7 cell line with empty pLEX (RAW-pLEX) was produced in the same manner as a negative control. Real-time quantitative reverse transcription PCR (RT-PCR) was performed to analyze the mRNA expression of TNF-a, IL-1 beta, NF kappa B-1 and NF kappa B-2 in S. aureus-stimulated RAW-Mdc-A2 cells and RAW-pLEX cells in untreated cells and cells treated for 3 h, 6 h, 12 h and 24 h. RTPCR was performed to analyze the mRNA expression of TNF-a, NFKB-1 and NF kappa B-2 stimulated by Lipoteichoic acid (LTA). Production of TNF-a was detected by enzyme-linked immunosorbent assay (ELISA). Colony counts were used to calculate the number of CFU per mL of cell culture supernatants. The results showed that compared to RAW-pLEX cells, stable transfection of Mdc-A2 in RAW264.7 cells stimulated by S. aureus significantly down-regulated the mRNA expression of TNF-a transcript variant 1 (TNF-a-tv-1) at 6 h and 12 h and the mRNA expression of TNF-a transcript variant 2 (TNF-a-tv-2) at 3 h, 6 h and 12 h. Compared to RAW-pLEX cells, stable transfection of Mdc-A2 in RAW264.7 cells stimulated by S. aureus significantly down -regulated the mRNA expression of IL-1 beta-T at 3 h, 6 h and 12 h as well as the mRNA expression of IL-1 beta at 3 h and 6 h. The expression and production of TNF-a and bacterial burden of cell culture supernatants were significantly down -regulated in RAW-Mdc-A2 cells stimulated by S. aureus, and the expression and production of TNF-a were significantly down -regulated in RAW-Mdc-A2 cells stimulated by LTA. Compared to RAW-pLEX cells, stable transfection of Mdc-A2 in RAW264.7 cells stimulated by S. aureus significantly down -regulated the mRNA expression of NF kappa B-1 at 3 h, 6 h and 12 h as well as the mRNA expression of NF kappa B-2 at 6 h. Additionally, stable transfection of Mdc-A2 in RAW264.7 cells stimulated by LTA significantly down -regulated the mRNA expression of NF kappa B-1. In conclusion, Mdc-A2 possesses potent anti-inflammatory activity and potent antimicrobial activity. Additionally, Mdc-A2 may interact with LTA and execute strong anti-inflammatory activity by blocking the activation of NF-kappa B signaling pathways. (C) 2017 Elsevier Ltd. All rights reserved.

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