The Effect of the Cyp19a1 Gene Methylation Modification on Temperature-dependent Sex Determination of Reeves' Turtle (Mauremys reevesii)

文献类型： 外文期刊

第一作者： Ru, Wenxiu

作者： Ru, Wenxiu;Chen, Hong;Lan, Xianyong;Ru, Wenxiu;Hua, Liushuai;Wei, Yufeng;Li, Weiye;Cao, Dainan;Ge, Yan;Gong, Shiping;Hua, Liushuai

作者机构：

关键词： Reeves' turtle;Mauremys reevesii;temperature;dependent sex determination (TSD);cyp19a1 gene;methylation

期刊名称：ASIAN HERPETOLOGICAL RESEARCH （ 影响因子：1.827； 五年影响因子：1.412 ）

ISSN： 2095-0357

年卷期： 2017 年 8 卷 3 期

页码：

收录情况： SCI

摘要： Temperature-dependent sex determination (TSD) is a type of environmental sex determination in which the sex of the embryos depends on the ambient temperature; however, the molecular mechanisms governing this process remain unknown. Aromatase, encoded by the cyp19a1 gene, which converts androgens into estrogens in animals, was considered to be the key gene for TSD. In this study, the 5'-flanking region of the cyp19a1 gene in Reeves' turtle (Mauremys reevesii) was cloned, and the promoter region was identified using the luciferase reporter assay. Then the eggs of Reeves' turtle were incubated at different temperatures (26 degrees C: male-biased temperature; 29 degrees C: non-sex-biased temperature and 32 degrees C: female-biased temperature). During the thermosensitive period, the adrenal kidney gonad complexes (AKG) were sampled. DNA methylation analysis of the AKG samples showed that the promoter region of the cyp19a1 gene was significantly de-methylated in the female-biased temperature regime (P<0.01). Quantitative analysis of the cyp19a1 gene and estrogen by qPCR and Elisa assay showed that the expression level of the cyp19a1 gene and estrogen content were both upregulated significantly at the female-biased temperature (P<0.01). These results indicated that the de-methylation response of the cyp19a1 gene to incubation temperature, especially at the female-biased temperature, could lead to temperature-specific expression of aromatase and increased estrogen levels, which may further determine gonadal development in Reeves' turtle. These findings provide insights into the genetic mechanisms underlying TSD.

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