Cloning and characterization of ovine immunoglobulin G Fc receptor III (Fc gamma RIII)

文献类型： 外文期刊

第一作者： Qiao, Song Lin

作者： Qiao, Song Lin;Chen, Yumei;Yang, Suzhen;Deng, Ruiguang;Zhang, Gaiping;Liu, Yunchao;Chang, Junbiao;Wang, Aiping;Chang, Junbiao;Chen, Yumei

作者机构：

关键词： Fc receptor;Sheep;Cloning;Expression

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）

ISSN： 0165-2427

年卷期： 2011 年 139 卷 2-4 期

页码：

收录情况： SCI

摘要： Receptors for the Fe regions of immunoglobin G (IgG) play a critical role in immunoregulation and immune defenses against pathogens. In this study, we describe the cloning, eukaryotic expression and IgG subclass specificity of ovine Fc gamma receptor III (Fc gamma RIII). The newly cloned ovine Fc gamma RIII cDNA contains a 940 bp open-reading frame (ORF), and is predicted to encode a 250 amino acid transmembrane glycoprotein composed of two immunoglobulin-like extracellular domains, a transmembrane region and a short cytoplasmic tail. The overall identity of the ovine Fc gamma RIII amino acid sequence to its cattle, pig and human counterparts was 83.2%, 62.0%, 60.7%, respectively. Overlapping PCR was performed with the extracellular domain of ovine Fc gamma RIII and the transmembrane and intracellular region of ovine Fc gamma chain to construct a chimeric receptor. Rosetting analysis showed that transfected COS-7 cells required Fc receptor gamma chain for the expression of Fc gamma RIII on the surface. COS-7 cells expressing Fc gamma RIII were able to bind chicken erythrocytes sensitized with ovine IgG1, but not IgG2. Identification of ovine Fc gamma RIII will further our understanding of the ovine immune system. (C) 2010 Elsevier B.V. All rights reserved.

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