Development of a Synthetic Peptide ELISA Assay for the Detection of Foot-and-Mouth Disease Virus Nonstructural Protein Antibodies

文献类型： 外文期刊

第一作者： Yang Ji-fei

作者： Yang Ji-fei;Yang Su-zhen;Yang Yan-yan;Zhi Ai-min;Zhao Dong;Zhi Yu-bao;Xing Guang-xu;Deng Rui-guang;Chai Shu-jun;Zhang Gai-ping;Yang Su-zhen

作者机构：

关键词： foot and mouth disease virus (FMDV);ELISA;synthetic peptide

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 11 期

页码：

收录情况： SCI

摘要： In order to develop an ELISA assay with synthetic peptides for the detection of antibody to the nonstructural proteins of foot-and-mouth disease virus, specific peptides were synthesized by a solid-phase method according to FMDV NSPs B-cell epitopes, and were conjugated to carrier protein BSA. An ELISA system was developed to detect FMDV NSPs antibody with the conjugated proteins as the coating antigen. The optimal coating concentration of the antigen was determined as 2.5 mu g mL(-1). The comparative study of this assay with UBI NSP ELISA kit and national commercial 3ABC ELISA kit in the detection of 199 serum samples showed that they were very coincident, and the identity rates were 96.48 and 97.48%, respectively. The development of ELISA using the synthetic peptides as coating antigen is specific, reproducible, stable, and easy, and can be used to differentiate FMDV infected pigs from immunized pigs.

分类号：

  • 相关文献

[1]Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice. Chen, Xuemei,Liu, Xiaodong,Ren, Xiuhua,Zang, Weidong,Li, Xuewu,Wang, Li. 2016

[2]Analysis of the linear epitope for Fc-binding on the mouse IgG Fc receptor (moFc gamma RI) by synthetic peptide. Wang, F. Y.,Guo, J. Q.,Zhang, G. P.. 2014

[3]Discovery and characterization of Coturnix chinensis avian ss-defensin 10, with broad antibacterial activity. Ma, Deying,Lin, Lijuan,Zhang, Kexin,Wang, Ruiqin,Lin, Lijuan,Zhang, Kexin,Han, Zongxi,Shao, Yuhao,Wang, Ruiqin,Liu, Shengwang. 2012

[4]Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or beta-galactosidase as a carrier of immunogenic epitopes. Li, GJ,Chen, WZ,Yan, WY,Zhao, K,Liu, MQ,Zhang, J,Fei, L,Xu, QX,Sheng, ZT,Lu, YG,Zheng, ZX.

[5]Expression of foot-and-mouth disease virus epitopes in tobacco by a tobacco mosaic virus-based vector. Wu, LG,Jiang, LB,Zhou, ZA,Fan, JH,Zhang, QQ,Zhu, HH,Han, Q,Xu, ZK.

[6]Polyclonal antibodies to grain gliadins and their application in wheat quality prediction. Li, Q. Y.,Song, X. Y.,Zhang, E. Y.,Pei, Y. H.,Li, Q. Y.,Ji, K. M.,Yan, Y. M.. 2008

[7]Quantification of toxins in a Cry1Ac+CpTI cotton cultivar and its potential effects on the honey bee Apis mellifera L.. Peng Han,Chang-Ying Niu,Chao-Liang Lei,Jin-Jie Cui,Nicolas Desneux. 2010

[8]Serological Survey of Canine Leptospirosis in Southern China. Liu, Mingchao,Liu, Jianzhu,Wang, Lin,Wang, Zhenyong,Wang, Shujing,Yang, Dubao,Chai, Tongjie,Shi, Dayou,Guo, Shining,Liao, Shenquan,Sun, Mingfei. 2012

[9]A monoclonal antibody-blocking enzyme-linked immunosorbent assay for the detection of serovar-specific antibodies to Haemophilus paragallinarum. Zhang, P,Blackall, PJ,Yamaguchi, T,Iritani, Y. 1999

[10]Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods. Romeis, Joerg,Wu, Kong-Ming,Peng, Yu-Fa,Romeis, Joerg. 2014

[11]Bioaccumulation of microcystin and its oxidative stress in the apple (Malus pumila). Chen, Jianzhong,Zhang, Haiyang,Wang, Chenyi,Zhou, Guoqing,Han, Zhiping,Chen, Jianzhong,Liu, Zhili,Dai, Jian. 2010

[12]Development and evaluation of a VP1-ELISA for detection of antibodies to duck hepatitis type 1 virus. Zhang, Tingting,Zhang, Yun,Meng, Fanyi,Li, Xiaojun,Kong, Xiangang,Liu, Ming,Hou, Zhenzhong,Feng, Xinchang. 2010

[13]Seroprevalence and Risk Factors of Fascioliasis in Yaks, Bos grunniens, from Three Counties of Gansu Province, China. Zhang, Xiao-Xuan,Ma, Jian-Gang,Zheng, Wen-Bin,Zhao, Quan,Zhang, Xiao-Xuan,Feng, Sheng-Yong,Ma, Jian-Gang,Zheng, Wen-Bin,Yin, Ming-Yang,Qin, Si-Yuan,Zhou, Dong-Hui,Zhu, Xing-Quan. 2017

[14]Preliminary serological evidence for Schmallenberg virus infection in China. Zhai, Shao-Lun,Lv, Dian-Hong,Wen, Xiao-Hui,Zhu, Xue-Liang,Yang, Yan-Qiu,Chen, Qin-Ling,Wei, Wen-Kang. 2018

[15]Seroprevalence of bovine theileriosis in northern China. Li, Yaqiong,Liu, Zhijie,Liu, Junlong,Yang, Jifei,Li, Qian,Guo, Pengfei,Guan, Guiquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Li, Youquan,Yin, Hong. 2016

[16]Identification of Theileria uilenbergi immunodominant protein for development of an indirect ELISA for diagnosis of ovine theileriosis. Liu, Zhijie,Kullmann, Birgit,Abdo, Jassim,Salih, Dialeldin,Ahmed, Jabbar,Seitzer, Ulrike,Liu, Zhijie,Wang, Zijian,Yin, Hong,Luo, Jianxun,Zhang, Bao. 2010

[17]Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay Based on Monoclonal Antibodies for the Detection of 2-Dodecylcyclobutanone in Irradiated Beef. Zhao, Yueliang,Wang, Feng,Li, Weiming,Ha, Yiming. 2013

[18]Development of Bt Rice and Bt Maize in China and Their Efficacy in Target Pest Control. Liu, Qingsong,Peng, Yufa,Li, Yunhe,Hallerman, Eric. 2016

[19]Development of an enzyme-linked immunosorbent assay for podophyllotoxin. Xu, DM,Yu, XY,Liu, YQ,Feng, JT,Pan, LG,Liu, XJ,He, J,Zhang, X. 2005

[20]Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON). Ji, Fang,Li, Hua,Xu, Jianhong,Shi, Jianrong. 2011

作者其他论文 更多>>

微信小程序