Verification and fine-mapping of QTLs conferring days to flowering in soybean using residual heterozygous lines

文献类型： 外文期刊

第一作者： Su ChengFu

作者： Su ChengFu;Lu WeiGuo;Zhao TuanJie;Gai JunYi;Su ChengFu;Lu WeiGuo;Zhao TuanJie;Gai JunYi;Su ChengFu;Lu WeiGuo;Zhao TuanJie;Gai JunYi;Lu WeiGuo

作者机构：

关键词： soybean;backcross line;RHL (residual heterozygous line);SSR (simple sequence repeat) marker;flowering date;QTL;fine-mapping

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2010 年 55 卷 6 期

页码：

收录情况： SCI

摘要： The results of QTL mapping based on a primary mapping population should be further verified and refined for its real utilization in marker-assisted selection or map-based cloning. The primary mapping population contains 114 BC(1)F(1) plants of the backcross between Essex (maturity group V, MG V) as the donor parent and ZDD2315 (MG II) as the recurrent parent. In this study, a genetic linkage map with 250 SSR markers spanning a total length of 2963.5 cM on 25 linkage groups (LG) was constructed using software MAPMAKER3.0. Six kinds of genetic statistical models of 4 softwares, i.e. WinQTL Cartographer Version 2.5, IciMapping Version 2.0, MapQTL Version 5.0 and QTLnetwork Version 2.0, were used to map QTLs conferring days to flowering of the BC(1)F(3) lines. Nine QTLs were mapped on 6 different linkage groups (LG). Of those, 6 QTLs were detected by at least two different genetic statistical models, while the other three were detected by only one procedure. Among the three QTLs, Flwdt7 was mapped between Sat_213 and Satt643 on LG C2 with only 11.0% contribution rate. For confirmation of Flwdt7, 5 RHL populations were developed through selfing eight BC(1)F(5) plants heterozygous at seven markers around the locus. The RHL populations with the same segregating loci were bulked and used to construct a secondary linkage map of the specific segment using software JoinMapA (R) 3.0. The genetic distances among the markers on the specific segment became shorter than those of the whole genome map. On the secondary map, Flwdt7 was mapped between Satt277 and Satt489, next to its primary interval Sat_213-Satt643, with distance 1.40 cM to Satt277 and 0.45 cM to Satt489, confidence interval narrowed to 2.7 cM, and contribution rate increased to 36.8%. The results were confirmed with significance analysis among marker genotypes on individual loci and comparison analysis of target marker intervals among near isogenic lines (plants). Thus the strategy by using residual heterozygous lines for QTL fine-mapping on target segments based on primary whole genome scanning with multiple mapping models was demonstrated to be effective.

分类号：

  • 相关文献

[1]High-resolution mapping and characterization of qRgls2, a major quantitative trait locus involved in maize resistance to gray leaf spot. Xu, Ling,Zhang, Yan,Zhu, Mang,Zhong, Tao,Xu, Mingliang,Shao, Siquan,Chen, Wei,Tan, Jing,Fan, Xingming. 2014

[2]QTL Mapping of Isoflavone, Oil and Protein Contents in Soybean (Glycine max L. Merr.). Liang Hui-zhen,Yu Yong-liang,Wang Shu-feng,Lian Yun,Wang Ting-feng,Wei Yan-li,Gong Peng-tao,Fang Xuan-jun,Liu Xue-yi,Zhang Meng-chen. 2010

[3]Identification of QTL underlying isoflavone contents in soybean seeds among multiple environments. Zeng, Guoliang,Li, Dongmei,Han, Yingpeng,Teng, Weili,Wang, Jian,Li, Wenbin,Qiu, Liquan. 2009

[4]Combining QTL and candidate gene analysis with phenotypic model to unravel the relationship between lodging and related traits in soybean. Chen, Haifeng,Yang, Zhonglu,Chen, Limiao,Zhang, Chanjuan,Yuan, Songli,Zhang, Xiaojuan,Qiu, Dezhen,Wan, Qiao,Chen, Shuilian,Shan, Zhihui,Zhou, Xinan,Zhan, Yong.

[5]Identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession PI 567516C. Jiao, Yongqing,Vuong, Tri D.,Liu, Yang,Li, Zenglu,Noe, Jim,Robbins, Robert T.,Joshi, Trupti,Xu, Dong,Shannon, J. Grover,Nguyen, Henry T.. 2015

[6]Validation of the quantitative trait locus underlying soybean plant height using residual heterozygous lines and near-isogenic lines across multi-environments. Yan, Long,Zhang, Yuanyuan,Yang, Chunyan,Chen, Qiang,Liu, Bingqiang,Di, Rui,Zhang, Mengchen,Yan, Long,Song, Qijian,Cregan, Perry B.,An, Yongqiang Charles,Wu, Chengjun.

[7]Identification of quantitative trait loci for leaf area and chlorophyll content in maize (Zea mays) under low nitrogen and low phosphorus supply. Cai, Hongguang,Chu, Qun,Yuan, Lixing,Liu, Jianchao,Chen, Xiaohui,Chen, Fanjun,Mi, Guohua,Zhang, Fusuo,Cai, Hongguang.

[8]Toward Identification of Black Lemma and Pericarp Gene Blp1 in Barley Combining Bulked Segregant Analysis and Specific-Locus Amplified Fragment Sequencing. Jia, Qiaojun,Liang, Zongsuo,Jia, Qiaojun,Liang, Zongsuo,Wang, Junmei,Zhu, Jinghuan,Hua, Wei,Shang, Yi,Yang, Jianming. 2017

[9]Characterization of a rice dwarf and narrow leaf 2 mutant. Adedze, Y. M. N.,Wei, X. J.,Sheng, Z. H.,Jiao, G. A.,Tang, S. Q.,Hu, P. S..

[10]Fine-mapping of qGW4.05, a major QTL for kernel weight and size in maize. Chen, Lin,Li, Yong-Xiang,Li, Chunhui,Wu, Xun,Qin, Weiwei,Li, Xin,Jiao, Fuchao,Zhang, Xiaojing,Zhang, Dengfeng,Shi, Yunsu,Song, Yanchun,Li, Yu,Wang, Tianyu. 2016

[11]A putative R3 MYB repressor is the candidate gene underlying atroviolacium, a locus for anthocyanin pigmentation in tomato fruit. Cao, Xue,Wang, Xiaotian,Van Giang, Tong,Liu, Xiaolin,Wang, Xiaoxuan,Gao, Jianchang,Guo, Yanmei,Du, Yongchen,Huang, Zejun,Qiu, Zhengkun,Wang, Jing,Wang, Guoping. 2017

[12]High-resolution mapping of brown planthopper (BPH) resistance gene Bph27(t) in rice (Oryza sativa L.). He, Jun,Liu, Yuqiang,Liu, Yanling,Jiang, Ling,Wu, Han,Kang, Haiyan,Liu, Shijia,Chen, Liangming,Liu, Xi,Cheng, Xianian,Wan, Jianmin,Wan, Jianmin.

[13]Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in Maize. Zhao, Yuanzeng,Lu, Xiaomin,Liu, Chaoxian,Guan, Haiying,Zhang, Mei,Li, Zhongfeng,Cai, Hongwei,Lai, Jinsheng,Zhao, Yuanzeng,Lu, Xiaomin,Liu, Chaoxian,Guan, Haiying,Zhang, Mei,Li, Zhongfeng,Cai, Hongwei,Lai, Jinsheng,Zhao, Yuanzeng,Lu, Xiaomin. 2012

[14]The genetic basic and fine-mapping of a stable quantitative-trait loci for aluminium tolerance in rice. Xue, Y.,Jiang, L.,Su, N.,Wang, J. K.,Deng, P.,Ma, J. F.,Zhai, H. Q.,Wan, J. M..

[15]Fine-mapping a fibre strength QTL QFS-D11-1 on cotton chromosome 21 using introgressed lines. Su, Chengfu,Yang, Liu,Li, Song,Wang, Wei,Pan, Qunbin,Qiu, Xinmian,Wang, Meixing.

[16]Regional association analysis-based fine mapping of three clustered QTL for verticillium wilt resistance in cotton (G. hirsutum. L). Yunlei Zhao,Wang, Hongmei,Hongmei Wang,Wei Chen,Pei Zhao,Haiyan Gong,Xiaohui Sang,Yanli Cui. 2017

[17]Quantitative trait loci mapping of adult-plant resistance to powdery mildew in Chinese wheat cultivar Lumai 21. Caixia Lan,Xiaowen Ni,Jun Yan,Yong Zhang,Xianchun Xia,Xinmin Chen,Zhonghu He.

[18]Characterization of a cell wall invertase gene TaCwi-A1 on common wheat chromosome 2A and development of functional markers. Dongyun Ma,Jun Yan,Zhonghu He,Ling Wu,Xianchun Xia.

[19]DISTRIBUTION OF SEVEN GRAIN GENES AND EVALUATION OF THEIR GENETIC EFFECTS ON GRAIN TRAITS. Zhang, Yadong,Zhao, Qingyong,Zhao, Chunfang,Chen, Tao,Zhu, Zhen,Zhou, Lihui,Yao, Shu,Zhao, Ling,Wang, Cailin. 2016

[20]QTL variations for growth-related traits in eight distinct families of common carp (Cyprinus carpio). Lv, Weihua,Yan, Yunqin,Lv, Weihua,Zheng, Xianhu,Kuang, Youyi,Cao, Dingchen,Sun, Xiaowen. 2016

作者其他论文 更多>>

微信小程序