Molecular cloning, tissue distribution and ontogenetic expression of sodium proton exchanger isoform 2 (NHE-2) mRNA in the small intestine of pigs

文献类型： 外文期刊

第一作者： Feng, D.

作者： Feng, D.;Wang, T.;Feng, D.;Zhi, A.;Zou, S.;Zhou, X.;Zuo, J.;Huang, Z.;Zhi, A.

作者机构：

关键词： ontogenetic expression;sodium proton exchanger (NHE-2);pigs

期刊名称：ANIMAL （ 影响因子：3.24； 五年影响因子：3.223 ）

ISSN： 1751-7311

年卷期： 2009 年 3 卷 3 期

页码：

收录情况： SCI

摘要： Molecular cloning, tissue distribution and ontogenetic regulation of sodium/proton exchanger isoform 2 (NHE-2) mRNA expression were evaluated in the pig small intestine during postnatal development The 2872-bp porcine full cDNA sequence of the NHE-2 (EF672046) cloned in this study showed 80% and 70% homology with known human and mouse gene sequence, respectively. Hydrophobic prediction suggests 13 putative membrane-spanning domains within porcine NHE-2. The porcine NHE-2 mRNA was detected in the brain, liver, kidney, heart, lung, small intestine and muscle. The small intestine had the highest NHE-2 mRNA abundance and the brain, lung and liver had the lowest NHE-2 mRNA abundance (P < 0.05). Along the longitudinal axis, the duodenum had the highest NHE-2 mRNA abundance and the ileum and colon had the lowest NHE-2 mRNA abundance (P < 0.05). The NHE-2 mRNA level was increased from day 1 to day 26 in the duodenum (P < 0.05) and dropped dramatically on day 30 (P < 0.05). There is no difference between day 1 and day 7 (P > 0.05). After day 30, the NHE-2 mRNA level remained the same except on day 90 (P > 0.05). The mRNA expression of NHE-2 was not only differentially regulated by age but also differentially distributed along the small intestine of piglets at early stages and growing Stages of life, which may contribute to changes in NHE activity.

分类号：

  • 相关文献

[1]Global copy number analyses by next generation sequencing provide insight into pig genome variation. Jiang, Jicai,Wang, Haifei,Kang, Huimin,Feng, Xiaotian,Zhang, Qin,Liu, Jian-Feng,Wang, Jiying,Zhang, Yan,Wang, Jiafu,Yin, Zongjun,Bao, Wenbin. 2014

[2]Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation. Lin, Haichao,Wang, Huaizhong,Wang, Yanping,Liu, Chang,Wang, Cheng,Guo, Jianfeng,Lin, Haichao,Wang, Yanping,Liu, Chang,Guo, Jianfeng. 2015

[3]Transcriptomic Analysis Identifies Candidate Genes and Gene Sets Controlling the Response of Porcine Peripheral Blood Mononuclear Cells to Poly I:C Stimulation. Wang, Jiying,Wang, Yanping,Wang, Huaizhong,Wu, Ying,Guo, Jianfeng,Wang, Haifei,Liu, Jian-Feng. 2016

[4]Dietary supplementation with a high dose of daidzein enhances the antioxidant capacity in swine muscle but experts pro-oxidant function in liver and fat tissues. Chen, Wei,Ma, Xianyong,Lin, Yingcai,Xiong, Yunxia,Zheng, Chuntian,Hu, Youjun,Yu, Deqian,Jiang, Zongyong,Chen, Wei,Ma, Xianyong,Lin, Yingcai,Xiong, Yunxia,Zheng, Chuntian,Hu, Youjun,Yu, Deqian,Jiang, Zongyong,Chen, Wei,Ma, Xianyong,Lin, Yingcai,Xiong, Yunxia,Zheng, Chuntian,Hu, Youjun,Yu, Deqian,Jiang, Zongyong,Chen, Wei,Ma, Xianyong,Lin, Yingcai,Xiong, Yunxia,Zheng, Chuntian,Hu, Youjun,Yu, Deqian,Jiang, Zongyong,Chen, Wei,Ma, Xianyong,Lin, Yingcai,Xiong, Yunxia,Zheng, Chuntian,Hu, Youjun,Yu, Deqian,Jiang, Zongyong. 2016

[5]High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China. Jiang, Hong-Xia,Chen, Zhang-Liu,Wang, Xiu-Mei,Chen, Ji-Rong,Liu, Ya-Hong,Liao, Xiao-Ping,Liu, Jian-Hua,Zeng, Zhen-Ling,Lue, Dian-Hong. 2011

[6]Performances of Different Fragment Sizes for Reduced Representation Bisulfite Sequencing in Pigs. Yuan, Xiao-Long,Zhang, Zhe,Pan, Rong-Yang,Gao, Ning,Deng, Xi,Li, Bin,Zhang, Hao,Li, Jia-Qi,Yuan, Xiao-Long,Sangild, Per Torp,Gao, Ning. 2017

[7]Metagenomic Analysis of Cecal Microbiome Identified Microbiota and Functional Capacities Associated with Feed Efficiency in Landrace Finishing Pigs. Tan, Zhen,Yang, Ting,Wang, Yuan,Xing, Kai,Zhang, Fengxia,Zhao, Xitong,Liu, Jianfeng,Wang, Chuduan,Ao, Hong,Chen, Shaokang. 2017

[8]Feeding brittle culm1 whole crop rice replacing wheat by-products to growing-finishing pigs. Wang, H. F.,Liu, J. X.,Qian, Q.,Wu, Y. M.. 2006

[9]Detection of differentially expressed genes in the longissimus dorsi of Northeastern Indigenous and Large White pigs. Gao, Y.,Jiang, H.,Wang, S.,Sun, G. J.,Li, F. J.,Deng, Q.,Dai, L. S.,Cui, X. S.,Liu, D. F.,Zhang, J. B.,Zhang, Y. H.,Ma, Q.,Zhao, Z. H.,Xiao, S. Q.,Zhang, S. M.. 2011

[10]Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation. Wang, Jiying,Wang, Yanping,Wang, Huaizhong,Hao, Xiaojing,Wu, Ying,Guo, Jianfeng. 2014

[11]Renaturation and purification of ApxII toxin of Actinobacillus pleuropneumoniae. Wang, Chunlai,Liu, Siguo,Peng, Yonggang,Shao, Meili,Wang, Yong,Gong, Qiang,Chang, Yuehong,Liu, Jiandong,Liu, Huifang,Liu, Di,Kong, Xiangang.

[12]High prevalence of bovine viral diarrhea virus 1 in Chinese swine herds. Deng, Yu,Sun, Chun-Qing,Lin, Tao,Yuan, Shi-Shan,Zhang, Hong-Biao,Zhai, Shao-Lun,Huang, Lv,Shan, Tong-Ling,Zheng, Hao,Tong, Guang-Zhi,Deng, Yu,Cao, San-Jie,Shan, Tong-Ling,Wen, Xin-Tian,Deng, Yu.

[13]Molecular characterization and expression analysis of the porcine caveolin-3 gene. Zhu, Zhengmao,Li, Yong,Mo, Delin,Li, Kui,Zhao, Shuhong.

[14]Fusion of C3d with hemagglutinin enhances protective immunity against swine influenza virus. Li, Guo-Xin,Tian, Zhi-Jun,Yu, Hai,Jin, Yuan-Yuan,Hou, Shao-Hua,Zhou, Yan-Jun,Liu, Tian-Qiang,Hu, Shou-Ping,Tong, Guang-Zhi,Li, Guo-Xin,Tong, Guang-Zhi,Li, Guo-Xin,Yu, Hai,Zhou, Yan-Jun,Tong, Guang-Zhi.

[15]Improved Detection and Characterization of Copy Number Variations Among Diverse Pig Breeds by Array CGH. Wang, Jiying,Jiang, Jicai,Wang, Haifei,Kang, Huimin,Zhang, Qin,Liu, Jian-Feng,Wang, Jiying. 2015

[16]Dose-dependent pathogenicity of a pseudorabies virus variant in pigs inoculated via intranasal route. Wang, Yimin,Xia, Shui-Li,Lei, Jian-Lin,Cong, Xin,Xiang, Guang-Tao,Luo, Yuzi,Sun, Yuan,Qiu, Hua-Ji.

[17]Mutation of the XIST gene upregulates expression of X-linked genes but decreases the developmental rates of cloned male porcine embryos. Yang, Yang,Wu, Dan,Liu, Dewu,Quan, Jianping,Cai, Gengyuan,Zheng, Enqin,Wu, Zhenfang,Li, Zicong,Yang, Yang,Wu, Dan,Liu, Dewu,Quan, Jianping,Cai, Gengyuan,Zheng, Enqin,Wu, Zhenfang,Li, Zicong,Shi, Junsong,Zhou, Rong,He, Xiaoyan.

[18]Evaluation of probiotic bacteria for their effects on the growth performance and intestinal microbiota of newly-weaned pigs fed fermented high-moisture maize. Wang, J. Q.,Yin, F. G.,Yu, H.,Gong, J.,Wang, J. Q.,Wang, J. Q.,Zhu, C.,Niven, S. J.,de Lange, C. F. M.,Gong, J.,Yin, F. G.. 2012

[19]Genetic parameters and genetic trends in the Chinese x European Tiameslan composite pig line. II. Genetic trends. Zhang, SQ,Bidanel, JP,Burlot, T,Legault, C,Naveau, J. 2000

[20]Genetic parameters and genetic trends in the Chinese x European Tiameslan composite pig line. I. Genetic parameters. Zhang, SQ,Bidanel, JP,Burlot, T,Legault, C,Naveau, J. 2000

作者其他论文 更多>>

微信小程序