Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage

文献类型： 外文期刊

第一作者： Chen, Jine

作者： Chen, Jine;Li, Fengbo;Liu, Yan;Shen, Weifeng;Du, Xin;He, Lihua;Meng, Zhiqi;Wang, Yongqiang;Ma, Xianliang

作者机构：

关键词： Bombyx mori;Midgut;Mitochondria;Posttranslational modification;Lysine succinylation;Metabolism

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

ISSN： 1874-3919

年卷期： 2018 年 174 卷

页码：

收录情况： SCI

摘要： Lysine succinylation is a newly identified protein post-translational modification (PTM) of lysine residues. Increasing evidences demonstrate that this modification is prevalent in mitochondria and regulates many vital cellular processes, especially metabolism. Here, we determined the succinylome of the silkworm (Bombyx mori) midgut mitochondria during the larval gluttonous stage (the fifth instar) using succinylated peptides enrichment coupled with nano HPLC/MS/MS. A total of 1884 lysine succinylation sites on 373 mitochondrial proteins were identified. The bioinformatic analysis reveal that succinylated proteins are significantly enriched in central metabolic processes and mitochondrial protein synthesis. Several apoptosis and detoxification related enzymes or proteins are succinylated. The findings suggest the crucial role of lysine succinylation in silkworm midgut metabolism and resistance. Our data provide a rich resource for further analysis of lysine succinylation in silkworm. Significance: Insect midgut is the vital tissue for nutrient metabolism and also for xenobiotic metabolism. There is a growing body of knowledge on regulation of midgut function at the gene or protein levels in silkworm, however, the regulation at post-translation modification level remains largely unknown. We provide a first global analysis of the mitochondrial lysine succinylome in silkworm midgut. A total of 1884 lysine succinylation sites on 373 mitochondrial proteins were identified. Bioinformatics results suggest an important role of this modification in regulating metabolism and mitochondrial protein synthesis. Our data greatly expand the catalog of lysine succinylation substrates and sites in insects, and represents an important resource for understanding the physiological function of lysine succinylation in insect midgut.

分类号：

  • 相关文献

[1]The physiological differentiation along the midgut of Bombyx mori - inspirations from proteomics and gene expression patterns of the secreted proteins in the ectoperitrophic space. Qu, M.,Yang, J.,Yang, Q.,Liu, L.,Qu, M.,Yang, J.,Yang, Q.,Yang, Q.. 2018

[2]Early responses of silkworm midgut to microsporidium infection - A Digital Gene Expression analysis. Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xiao, Sheng-Yan,Li, Nan,Shen, Zhong-Yuan.

[3]Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Deng, Xiangyuan.

[4]Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori. Wu, Ping,Li, Mu-Wang,Guo, Xi-Jie,Jiang, Yun-Feng,Wang, Zi-Sheng,Guo, Xi-Jie. 2010

[5]Differentially expressed genes in the midgut of Silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Li, Mu-Wang,Wang, Xiu-Ye,Liu, Ting,Qin, Guang-Xing,Guo, Xi-Jie,Wang, Xiu,Zhao, Pan,Guo, Xi-Jie. 2009

[6]Novel protein of IBP from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan. 2012

[7]Digital Gene Expression analysis in the midgut of 4008 silkworm strain infected with cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie. 2014

[8]iTRAQ-based quantitative proteomic analysis of midgut in silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-Yuan,Shang, Meng-Ke,Qin, Guang-Xing,Hou, Cheng-Xiang,Guo, Xi-Jie.

[9]Autophagy precedes apoptosis during the remodeling of silkworm larval midgut. Franzetti, Eleonora,Grimaldi, Annalisa,Tettamanti, Gianluca,Huang, Zhi-Jun,Shi, Yan-Xia,Xie, Kun,Deng, Xiao-Juan,Li, Jian-Ping,Li, Qing-Rong,Yang, Wan-Ying,Zeng, Wen-Nian,Cao, Yang,Li, Qing-Rong,Casartelli, Morena,Deng, Hui-Min,Feng, Qili,Cappellozza, Silvia,Xia, Qingyou.

[10]Towards posttranslational modification proteome of royal jelly. Zhang, Lan,Fang, Yu,Li, Rongli,Feng, Mao,Han, Bin,Zhou, Tiane,Li, Jianke.

[11]Down-regulation of aminopeptidase N and ABC transporter subfamily G transcripts in Cry1Ab and Cry1Ac resistant Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). Wang, Yueqin,Wang, Yidong,Bai, Shuxiong,Wang, Zhenying,He, Kanglai,Coates, Brad S.. 2017

[12]Activities of Digestive Enzymes in the Omnivorous Pest Apolygus lucorum (Hemiptera: Miridae). Yuan, Wei,Wu, Kongming,Zhao, Xincheng.

[13]Reduction of Bacillus thuringiensis Cry1Ac toxicity against Helicoverpa armigera by a soluble toxin-binding cadherin fragment. Liu, Chenxi,Wu, Kongming,Gao, Yulin,Ning, Changming,Wu, Yidong,Ning, Changming,Oppert, Brenda.

[14]Tissue-specific transcriptome profiling of Plutella xylostella third instar larval midgut. Xie, Wen,Lei, Yanyuan,Fu, Wei,Yang, Zhongxia,Zhu, Xun,Guo, Zhaojiang,Wu, Qingjun,Wang, Shaoli,Xu, Baoyun,Zhang, Youjun,Zhou, Xuguo.

[15]Analysis of midgut gene expression profiles from different silkworm varieties after exposure to high temperature. Li, Qing Rong,Xiao, Yang,Wu, Fu Quan,Ye, Ming Qiang,Luo, Guo Qing,Xing, Dong Xu,Li, Li,Yang, Qiong.

[16]Sulfide-based ATP production in Urechis unicinctus. Ma Zhuojun,Bao Zhenmin,Wang Sifeng,Zhang Zhifeng,Ma Zhuojun,Wang Sifeng. 2010

[17]Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed. Xin, Xia,Tian, Qian,Yin, Guangkun,Chen, Xiaoling,Zhang, Jinmei,Lu, Xinxiong,Tian, Qian,Ng, Sophia,Ng, Sophia. 2014

[18]Mechanism of Free Zn(2+) Enhancing Inhibitory Effects of EGCG on the Growth of PC-3 Cells: Interactions with Mitochondria. Shen, Shengrong,Yu, Haining,Sun, Shili,Das, Undurti N.. 2009

[19]Nitric oxide suppresses aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips by improving mitochondrial physiological properties. Huang, Wenjing,Oo, Thet Lwin,Gu, Minghua,Zhan, Jie,Wang, Aiqin,He, Long-Fei,He, Huyi,He, Long-Fei. 2018

[20]Transport of Calcium Ions into Mitochondria. Xu, Zhaolong,Zhang, Dayong,He, Xiaolan,Huang, Yihong,Shao, Hongbo,Shao, Hongbo. 2016

作者其他论文 更多>>

微信小程序