Purification and Characterization of Antiproliferative Peptide from Enzymatic Hydrolysates of Chinese Soft-Shelled Turtle Protein
文献类型: 外文期刊
第一作者: Wang, Nan
作者: Wang, Nan;Liu, Caiqin;Jin Jiancang;Lu, Yin;Wang, Wei;Liu, Xiaoxiang
作者机构:
关键词: Antiproliferative activity;Peptide;Chinese soft-shelled turtle;Enzymatic hydrolysis;Q-TOF MS/MS
期刊名称:INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY ( 影响因子:0.822; 五年影响因子:0.906 )
ISSN: 1560-8530
年卷期: 2018 年 20 卷 1 期
页码:
收录情况: SCI
摘要: Chinese soft-shelled turtle protein was hydrolyzed using neutrase to produce antiproliferative peptides. To identify the antiproliferative peptides, Chinese soft-shelled turtle protein hydrolysate was fractionated by Sephadex G-25. Antiproliferative activities of the identified peptides were evaluated using the MTT assay. Three novel antiproliferative peptides were identified as Asp-Glu-Gla-Asp-Leu-Leu-Gla (MW: 745.79 Da), Glu-Gla-Gly-Val-Asn-Asp-Trp (MW: 789.81 Da), and Gly-Ger-IleGer-Ger-Gly-Gln-Val (MW: 733.78 Da). These peptides with exhibited antiproliferative activity on A549 cancer cells with IC50 values of 129.12 +/- 4.21, 150.89 +/- 3.75 and 142.47 +/- 5.12 mu g/mL, respectively. This bioactivity was likely due to the small size of the peptides and the peptide sequences have hydrophobic amino acids. Further investigations were suggested on larger scale employing standard procedures. (C) 2018 Friends Science Publishers
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