Purification and Characterization of Antiproliferative Peptide from Enzymatic Hydrolysates of Chinese Soft-Shelled Turtle Protein

文献类型: 外文期刊

第一作者: Wang, Nan

作者: Wang, Nan;Liu, Caiqin;Jin Jiancang;Lu, Yin;Wang, Wei;Liu, Xiaoxiang

作者机构:

关键词: Antiproliferative activity;Peptide;Chinese soft-shelled turtle;Enzymatic hydrolysis;Q-TOF MS/MS

期刊名称:INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY ( 影响因子:0.822; 五年影响因子:0.906 )

ISSN: 1560-8530

年卷期: 2018 年 20 卷 1 期

页码:

收录情况: SCI

摘要: Chinese soft-shelled turtle protein was hydrolyzed using neutrase to produce antiproliferative peptides. To identify the antiproliferative peptides, Chinese soft-shelled turtle protein hydrolysate was fractionated by Sephadex G-25. Antiproliferative activities of the identified peptides were evaluated using the MTT assay. Three novel antiproliferative peptides were identified as Asp-Glu-Gla-Asp-Leu-Leu-Gla (MW: 745.79 Da), Glu-Gla-Gly-Val-Asn-Asp-Trp (MW: 789.81 Da), and Gly-Ger-IleGer-Ger-Gly-Gln-Val (MW: 733.78 Da). These peptides with exhibited antiproliferative activity on A549 cancer cells with IC50 values of 129.12 +/- 4.21, 150.89 +/- 3.75 and 142.47 +/- 5.12 mu g/mL, respectively. This bioactivity was likely due to the small size of the peptides and the peptide sequences have hydrophobic amino acids. Further investigations were suggested on larger scale employing standard procedures. (C) 2018 Friends Science Publishers

分类号:

  • 相关文献

[1]Isolation and Characterization of a Novel Lectin from the Edible Mushroom Stropharia rugosoannulata. Zhang, Weiwei,Geng, Xueran,Wang, Hexiang,Zhang, Weiwei,Geng, Xueran,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Tzi Bun Ng,Zhao, Liyan.

[2]A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea. Hu, D. D.,Zhang, G. Q.,Wang, H. X.,Hu, D. D.,Zhang, G. Q.,Wang, H. X.,Zhang, R. Y.,Ng, T. B.. 2011

[3]Evaluation of the protein requirement of juvenile Chinese soft-shelled turtle (Pelodiscus sinensis, Wiegmann) fed with practical diets. Wang, Jun,Yang, Zhencai,Wang, Jun,Qi, Zhanhui.

[4]Comparative study of two immunity-related GTPase genes in Chinese soft-shell turtle reveals their molecular characteristics and functional activity in immune defense. Shi, Yan,Hu, Sufei,Ding, Tie,Zhao, Zhe,Shi, Yan,Shen, Sixian,Hong, Xiaoyou,Chen, Chen,Zhu, Xinping. 2018

[5]Modern greenhouse culture of juvenile soft-shelled turtle, Pelodiscus sinensis. Zhang, Jing,Wang, Fen,Jiang, Ye-Lin,Hou, Guan-Jun,Cheng, Yun-Sheng,Chen, Hong-Lian,Zhang, Jing,Wang, Fen,Jiang, Ye-Lin,Hou, Guan-Jun,Cheng, Yun-Sheng,Chen, Hong-Lian,Li, Xiang,Li, Xiang.

[6]Phage-borne peptidomimetics as immunochemical reagent in dot-immunoassay for mycotoxin zearalenone. He, Qing-hua,Xu, Yang,Li, Yan-ping,Huang, Zhi-bing,Zhang, Cun-zheng. 2014

[7]Immunomodulating Effects of The Peptides Derived from Whey Protein. Miao Yuping,Liu Zhidong,Fan Wenya,Qu Yinhong,Gong Hunyu. 2012

[8]Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus-infected pigs from vaccinated pigs. Yang, Suzhen,Yang, Jifei,Zhang, Gaiping,Qiao, Songlin,Zhao, Dong,Li, Xuewu,Deng, Ruiguang,Zhi, Aimin,You, Leiming,Chai, Sujun,Teng, Man,Yang, Suzhen,Wang, Xuannian. 2010

[9]Immunomodulatory activities of a new pentapeptide (Bursopentin) from the chicken bursa of Fabricius. Li, D. Y.,Chen, P. Y.,Geng, Z. R.,Zhu, H. F.,Miao, D. N.,Wang, C..

[10]Antifungal peptide produced by Paenibacillus polymyxa BRF-1 isolated from soybean rhizosphere. Chen, Xueli,Wang, Guanghua,Xu, Meina,Jin, Jian,Liu, Xiaobing,Chen, Xueli. 2010

[11]Purification and Identification of an ACE Inhibitory Peptide from Walnut Protein. Liu, Meng,Du, Ming,Zhang, Yingchun,Xu, Weili,Zhang, Lanwei,Wang, Cong,Wang, Kejian.

[12]Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases. Wu, Ribang,Liu, Dan,Huang, Jiafeng,Zhang, Jiang,Xiao, Xiao,Lei, Ming,Chen, Yuelin,He, Hailun,Chen, Leilei,Chen, Leilei. 2017

[13]High pressure assist-alkali pretreatment of cotton stalk and physiochemical characterization of biomass. Du, Shuang-kui,Zhu, Xinna,Wang, Hua,Zhou, Dayun,Yang, Weihua,Xu, Hongxia.

[14]Enhanced Enzymatic Hydrolysis of Poplar after Combined Dilute NaOH and Fenton Pretreatment. Zhang, Chunyan,Wang, Shanshan,Cui, Zhongyi,Liu, Ping,Pei, Haisheng. 2016

[15]Development of the cuticular membrane and biomechanical properties in Hupingzao (Ziziphus jujuba Mill. 'Hupingzao'). Li, Na,Song, Yuqin,Chen, Yuanyuan,Li, Liulin,Li, Jie,Xue, Xiaofang. 2018

[16]Properties of Klebsiella phage P13 and associated exopolysaccharide depolymerase. Liu Yang,Chai Zihan,Shang Anqi,Mou Haijin,Li Guiyang,Mo Zhaolan. 2014

[17]ENZYMATIC-HYDROLYSIS OF WHEAT-STRAW IRRADIATED BY ELECTRON-BEAM IN PRESENCE OF PERACETIC-ACID SOLUTION. LU, ZX,KUMAKURA, M. 1995

[18]Effects of different enzymatic hydrolysis methods on the bioactivity of peptidoglycan in Litopenaeus vannamei. Song Xiaoling,Zhang Yue,Wei Song,Huang Jie,Zhang Yue. 2013

[19]Effect of Ionic Liquid Pretreatment on the Structure and Enzymatic Saccharification of Cassava Stillage Residues. Cui, Lihong,Li, Jihua,Wang, Qinghuang,Wang, Meng. 2014

[20]Preliminary evaluation of five elephant grass cultivars harvested at different time for sugar production. Li, Yuanyuan,Zhang, Yelong,Zheng, Hongbo,Zhang, Hongman,Du, Jian,Huang, He,Wu, Juanzi. 2015

作者其他论文 更多>>