Determination of gamma-hydroxybutyrate in human urine samples by ion exclusion and ion exchange two-dimensional chromatography system

文献类型： 外文期刊

第一作者： Liu, Junwei

作者： Liu, Junwei;Wang, Guoqing;Sun, Yu-an;Deng, Zhifen;Zhu, Zuoyi;Wang, Yong;Zhu, Yan

作者机构：

关键词： gamma-hydroxybutyrate;Two-dimensional chromatography;Ion exclusion;Ion exchange;Column switching

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）

ISSN： 0021-9673

年卷期： 2017 年 1528 卷

页码：

收录情况： SCI

摘要： A two-dimensional ion chromatography system was developed for the determination of gamma-hydroxybutyrate (GHB) in human urine samples. Ion exclusion chromatography was used in the first dimensional separation for elimination of urine matrices and detection of GHB above 10 mg L-1, ion exchange chromatography was used in the second dimensional separation via column-switching technique for detection of GHB above 0.08 mgL(-1). Under the optimized chromatographic conditions, the ion exclusion and ion exchange chromatography separation system exhibited satisfactory repeatability (RSD < 3.01%, n=6) and good linearity in the range of 50-1000 mg L-1 and 0.5-100 mg L-1, respectively. By this method, concentrations of GHB in the selected human urine samples were detected in the range of 0-1.57 mg L-1 . The urine sample containing 0.89 mg L-1 GHB was selected to evaluate the accuracy; the spiked recoveries of GHB were 95.9-102.8%. The results showed that the two-dimensional ion chromatography system was convenient and practical for the determination of GHB in human urine samples. (C) 2017 Elsevier B.V. All rights reserved.

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