Development and validation of a functional co-dominant SNP marker for the photoperiod thermo-sensitive genic male sterility pms3 (p/tms12-1) gene in rice

文献类型： 外文期刊

第一作者： Qi, Yongbin

作者： Qi, Yongbin;Wang, Linyou;Gui, Junmei;Zhang, Lixia;Liu, Qinglong;Wang, Jianjun

作者机构：

关键词： Oryza sativa;photoperiod-thermo-sensitive genic male sterility (P/TGMS);SNP;MAS;functional marker

期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）

ISSN： 1344-7610

年卷期： 2017 年 67 卷 5 期

页码：

收录情况： SCI

摘要： The fertility conversion of photoperiod thermo-sensitive genic male sterility (P/TGMS) lines in rice is mostly regulated by the P/TGMS genes in different environmental conditions. A point mutation with G-C on the pms3 (p/tms12-1) gene regulates the pollen fertility of Nongken58S and a large amount of Nongken58S-derived lines. In this study, we developed and designed a functional co-dominant marker according to the SNP loci for the pms3 (p/tms12-1) gene. We can differentiate the SNP loci in pms3 (p/tms12-1) gene from another TGMS lines and inbred cultivars using the dpms3-54 marker. The results showed that 376 bp band was detected in the homozygous genotype for pms3 (p/tms12-1), while 359 bp band was detected in the homozygous genotype for non pms3 (p/trns12-1), two bands with 376 and 359 bp were detected in the heterozygous genotype. The dpms3-54 marker can be used to test the purity of two-line hybrid rice seeds and to divide each of F-1 plant into homozygous and heterozygous genotypes at the seedling stage. Thus, this study provide a useful functional marker to detect pms3 (p/tms12-1) gene in different genetic resources and populations, which can be applied to the development and breeding of P/TGMS lines in two-line hybrid rice system with marker-assisted selection.

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