Online monitoring of astragaloside II metabolism using a homemade cultural device coupled with microdialysis and ultra-performance liquid chromatography mass spectrometry
文献类型: 外文期刊
第一作者: Li, Xue
作者: Li, Xue;Pi, Zifeng;Liu, Shu;Liu, Zhiqiang;Song, Fengrui;Li, Xue;Wang, Wei;Li, Xue;Wang, Wei
作者机构:
关键词: Astragaloside;Cyclodextrin;Intestinal microbiota;Microdialysis;Ultra-performance liquid;chromatography mass spectrometry
期刊名称:JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ( 影响因子:3.205; 五年影响因子:3.068 )
ISSN: 1570-0232
年卷期: 2017 年 1063 卷
页码:
收录情况: SCI
摘要: A new system was described for the online monitoring of astragaloside II (AII) metabolism in intestinal microbial community. The system was based on a homemade cultural device coupled with microdialysis (MD) and ultra performance liquid chromatography-mass spectrometry (UPLC-MS). Main improvements include a simplified anaerobic incubator enabling the experiment to be conducted in ambient atmosphere, continuous sampling, and decreased matrix effect. Importantly, our method distinctly decreases the interference of small molecules by adding 20 mg ml(-1) of 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) to the perfusion fluid. Using the developed method, the metabolism of All in intestinal bacteria was successfully investigated. Results were then compared with those obtained by conventional incubation and sampling method. We found that the integrated experimental system maintained the proper fermentation environment for bacteria and enabled high chromatography performance. With the advantages of auto-sampling, online detection, non-requirement of expensive fermenting equipment, and negligible matrix interference, the method can greatly contribute to the investigation of the dynamic biotransformation of astragalosides in complicated matrix-based biological samples.
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