Genome characterization of a novel binary toxin-positive strain of Clostridium difficile and comparison with the epidemic 027 and 078 strains

文献类型： 外文期刊

第一作者： Liu, Sidi

作者： Liu, Sidi;Meng, Xiujuan;Duan, Juping;Fu, Chenchao;Wu, Anhua;Li, Chunhui;Peng, Zhong;Xu, Zhuofei;Wu, Bin;Liang, Wan;Tang, Biao;Wang, Yuanguo;Duan, Juping

作者机构：

关键词： Clostridium difficile;ST201;Binary toxin-positive;Whole genome sequencing;Comparative genomic analysis

期刊名称：GUT PATHOGENS （ 影响因子：4.181； 五年影响因子：4.524 ）

ISSN： 1757-4749

年卷期： 2017 年 9 卷

页码：

收录情况： SCI

摘要： Background: Clostridium difficile is an anaerobic Gram-positive spore-forming gut pathogen that causes antibiotic-associated diarrhea worldwide. A small number of C. difficile strains express the binary toxin (CDT), which is generally found in C. difficile 027 (ST1) and/or 078 (ST11) in clinic. However, we isolated a binary toxin-positive non-027, non-078 C. difficile LC693 that is associated with severe diarrhea in China. The genotype of this strain was determined as ST201. To understand the pathogenesis-basis of C. difficile ST201, the strain LC693 was chosen for whole genome sequencing, and its genome sequence was analyzed together with the other two ST201 strains VL-0104 and VL-0391 and compared to the epidemic 027/ST1 and 078/ST11 strains. Results: The project finally generated an estimated genome size of approximately 4.07 Mbp for strain LC693. Genome size of the three ST201 strains ranged from 4.07 to 4.16 Mb, with an average GC content between 28.5 and 28.9%. Phylogenetic analysis demonstrated that the ST201 strains belonged to clade 3. The ST201 genomes contained more than 40 antibiotic resistance genes and 15 of them were predicted to be associated with vancomycin-resistance. The ST201 strains contained a larger PaLoc with a Tn6218 element inserted than the 027/ST1 and 078/ST11 strains, and encoded a truncated TcdC. In addition, the ST201 strains contained intact binary toxin coding and regulation genes which are highly homologous to the 027/ST1 strain. Genome comparison of the ST201 strains with the epidemic 027 and 078 strain identified 641 genes specific for C. difficile ST201, and a number of them were predicted as fitness and virulence associated genes. The presence of those genes also contributes to the pathogenesis of the ST201 strains. Conclusions: In this study, the genomic characterization of three binary toxin-positive C. difficile ST201 strains in clade 3 was discussed and compared to the genomes of the epidemic 027 and the 078 strains. Our analysis identified a number fitness and virulence associated genes/loci in the ST201 genomes that contribute to the pathogenesis of C. difficile ST201.

分类号：

  • 相关文献

[1]Equine hyperimmune serum protects mice against Clostridium difficile spore challenge. Yan, Weiwei,Shin, Kang-Soon,Wang, Shih-Jon,Xiang, Hua,Akey, Bruce,Mohamed, Hussni,Chang, Yung-Fu,Divers, Thomas,McDonough, Sean,Wang, Shih-Jon,Xiang, Hua,Bowman, James,Rowlands, Anne.

[2]The recombinant Lactococcus lactis oral vaccine induces protection against C. difficile spore challenge in a mouse model. Yan, Weiwei,McDonough, Sean P.,Lin, Nengfeng,Wu, Katherine J.,He, Hongxuan,Xiang, Hua,Yang, Maosheng,Moreira, Maira Aparecida S.,Chang, Yung-Fu,Guo, Shanguang,He, Hongxuan,Xiang, Hua,Yang, Maosheng,Moreira, Maira Aparecida S..

[3]Comparative genomics unravels metabolic differences at the species and/or strain level and extremely acidic environmental adaptation of ten bacteria -belonging to the genus Acidithiobacillus. Zhang, Xian,She, Siyuan,Dong, Weiling,Niu, Jiaojiao,Xiao, Yunhua,Liang, Yili,Liu, Xueduan,Yin, Huaqun,Zhang, Xian,She, Siyuan,Dong, Weiling,Niu, Jiaojiao,Xiao, Yunhua,Liang, Yili,Liu, Xueduan,Yin, Huaqun,Zhang, Xiaoxia,Zhang, Xiaoxia,Fan, Fenliang,Fan, Fenliang.

[4]Comparative genomic analysis between newly sequenced Brucella suis Vaccine Strain S2 and the Virulent Brucella suis Strain 1330. Di, Dong-dong,Tian, Li-li,Kang, Jing-li,Ni, Bo,Fan, Wei-xing,Mao, Xiang,Jiang, Hai,Zhang, Wen,Cui, Bu-yun,Yi, Xin-ping,Ye, Feng,Zhong, Qi,He, You-yu,Xia, Lin,Yu, Yao. 2016

[5]Systematic Profiling of Short Tandem Repeats in the Cattle Genome. Xu, Lingyang,Li, Junya,Xu, Lingyang,Zhou, Yang,Bickhart, Derek M.,Sonstegard, Tad S.,Van Tassell, Curtis P.,Liu, George E.,Xu, Lingyang,Song, Jiuzhou,Haasl, Ryan J.,Sun, Jiajie,Zhou, Yang,Sonstegard, Tad S.,Lewin, Harris A.. 2017

[6]Genome-wide analysis of Dongxiang wild rice (Oryza rufipogon Griff.) to investigate lost/acquired genes during rice domestication. Zhang, Fantao,Luo, Xiangdong,Xie, Jiankun,Xu, Tao,Mao, Linyong,Yan, Shuangyong,Chen, Xiwen,Gao, Shan,Wu, Zhenfeng,Chen, Rui. 2016

[7]Whole Genome Sequencing of Hulunbuir Short-Tailed Sheep for Identifying Candidate Genes Related to the Short-Tail Phenotype. Zhi, Dafu,Liu, Moning,Cheng, Chen,Wang, Xin,Li, Xiunan,Wei, Wei,Cao, Guifang,Zhang, Yukun,Tian, Zhipeng,Long, Xin,Da, Lai,Yang, Yanyan,He, Tingyi. 2018

[8]Comparative genomic analysis of the IId subtype family of Cryptosporidium parvum. Feng, Yaoyu,Li, Na,Feng, Yaoyu,Liu, Guangyuan,Li, Na,Roellig, Dawn M.,Kelley, Alyssa,Amer, Said,Xiao, Lihua,Amer, Said,Xiao, Lihua,Zhang, Longxian.

[9]Draft genome of the reindeer (Rangifer tarandus). Li, Zhipeng,Ba, Hengxing,Li, Guangyu,Lin, Zeshan,Chen, Lei,Yang, Yongzhi,Wang, Kun,Qiu, Qiang,Wang, Wen. 2017

[10]Identification of Genomic Insertion and Flanking Sequence of G2-EPSPS and GAT Transgenes in Soybean Using Whole Genome Sequencing Method. Guo, Bingfu,Guo, Yong,Hong, Huilong,Qiu, Li-Juan,Guo, Bingfu,Guo, Yong,Hong, Huilong,Qiu, Li-Juan,Hong, Huilong. 2016

[11]Latest Advances in Watermelon Genomics. Guo, Shaogui,Xu, Yong,Zhang, Haiying,Gong, Guoyi,Guo, Shaogui,Zheng, Yi,Fei, Zhangjun,Huang, Sanwen,Yi, Hongping,Wu, Mingzhu,Fei, Zhangjun,Zheng, Yi. 2010

[12]A detailed analysis of the recombination landscape of the button mushroom Agaricus bisporus var. bisporus. Sonnenberg, Anton S. M.,Gao, Wei,Lavrijssen, Brian,Hendrickx, Patrick,Sedaghat-Tellgerd, Narges,Baars, Johan J. P.,Visser, Richard G. F.,Foulongne-Oriol, Marie,Kong, Won-Sik,Schijlen, Elio G. W. M.,Gao, Wei.

[13]Genome sequencing and analysis of Kloeckera apiculata strain 34-9, a biocontrol agent against postharvest pathogens in citrus. Chen, Kai,Zheng, Feng,Long, Chao-an,Yang, Xiaoping.

作者其他论文 更多>>

微信小程序