Transgenic Eimeria magna Perard, 1925 Displays similar Parasitological Properties to the Wild-type strain and induces an exogenous Protein-specific immune response in rabbits (Oryctolagus cuniculus L.)

文献类型： 外文期刊

第一作者： Tao, Geru

作者： Tao, Geru;Tang, Xinming;Wang, Yunzhou;Li, Chao;Suo, Jingxia;Tian, Xiuling;Liu, Xianyong;Suo, Xun;Tao, Geru;Tang, Xinming;Wang, Yunzhou;Li, Chao;Suo, Jingxia;Tian, Xiuling;Liu, Xianyong;Suo, Xun;Tao, Geru;Tang, Xinming;Wang, Yunzhou;Li, Chao;Suo, Jingxia;Tian, Xiuling;Liu, Xianyong;Suo, Xun;Shi, Tuanyuan;Duszynski, Donald W.

作者机构：

关键词： rabbit coccidia;transgenic coccidia;Eimeria magna;life cycle;immunity

期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：7.561； 五年影响因子：7.624 ）

ISSN： 1664-3224

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Rabbit coccidiosis causes great economic losses to world rabbitries. Little work has been done considering genetic manipulation on the etiological agents, rabbit Eimeria spp. In this study, we constructed a transgenic line of Eimeria magna (EmagER) expressing enhanced yellow fluorescent protein (EYFP) and red fluorescent protein (RFP) using regulatory sequences of Eimeria tenella and Toxoplasma gondii. We observed the life cycle of EmagER and confirmed that the transgenic parasites express exogenous proteins targeted to different cellular compartments throughout the entire life cycle. EYFP was expressed mainly in the nucleus and RFP both in the nucleus and cytoplasm. Then, coccidia-free, laboratory-reared 40-day-old rabbits were primarily infected with either EmagER or wild-type strain oocysts and challenged with the wild-type strain. EmagER showed similar reproductivity and immunogenicity to the wild-type strain. Finally, we examined the foreign protein-specific immune response elicited by EmagER. Rabbits were immunized with either transgenic or wild-type oocysts. Immune response against parasite-soluble antigen, EYFP and RFP in spleen, and mesenteric lymph nodes were detected by quantitative real-time PCR. The relative expression level of IFN-alpha, IL-2, and TNF-alpha were higher in EmagER-immunized rabbits than wild-type parasites-immunized rabbits after stimulation with EYFP and RFP. Our study confirmed that a specific immune response was induced by the exogenous protein expressed by EmagER and favored future studies on application of transgenic rabbit coccidia as recombinant vaccine vectors.

分类号：

  • 相关文献

[1]The complete mitochondrial genome sequence of Eimeria magna (Apicomplexa: Coccidia). Tian, Si-Qin,Wang, Chun-Ren,Zhu, Xing-Quan,Tian, Si-Qin,Liu, Guo-Hua,Zhu, Xing-Quan,Cui, Ping,Fang, Su-Fang,Liu, Guo-Hua,Zhu, Xing-Quan. 2015

[2]Wheat stripe (yellow) rust caused by Puccinia striiformis f. sp.tritici. Chen, Wanquan,Liu, Taiguo,Wellings, Colin,Chen, Xianming,Chen, Xianming,Kang, Zhengsheng. 2014

[3]Are adult life history traits in oriental fruit moth affected by a mild pupal heat stress?. Cheng, Xiongbin,Zhang, Bo,Ma, Chun-Sen,Hoffmann, Ary A..

[4]The life cycle and pathogenicity of Eimeria fulva Farr, 1953 in domestic goslings. Dai, YB,Liu, M,He, NY,Tao, JP,Liu, XY.

[5]Stable Transfection of Eimeria intestinalis and Investigation of Its Life Cycle, Reproduction and Immunogenicity. Shi, Tuanyuan,Bao, Guolian,Fu, Yuan,Tao, Geru,Suo, Jingxia,Suo, Xun,Hao, Lili. 2016

[6]The life cycle of Haemaphysalis qinghaiensis (Acari: Ixodidae) ticks under laboratory conditions. Ma, Miling,Guan, Guiquan,Chen, Ze,Liu, Zhijie,Liu, Aihong,Gou, Huitian,Ren, Qiaoyun,Li, Youquan,Niu, Qingli,Yang, Jifei,Yin, Hong,Luo, Jianxun. 2013

[7]Assaying the potential of twenty-one legume plants in Medicago truncatula and M-sativa for candidate model plants for investigation the interactions with Heterodera glycines. Wu Du-qing,Huang Wen-kun,Peng Huan,He Wen-ting,Peng De-liang. 2016

[8]The role of Ustilaginoidea virens sclerotia in increasing incidence of rice false smut disease in the subtropical zone in China. Yong, Mingli,Deng, Qide,Fan, Linlin,Wang, Zhengyi,Hu, Dongwei,Miao, Jiankun,Wang, Shu,Bai, Yuanjun,Lai, Chaohui,Chen, Hongming,Yang, Xiujuan,Chen, Furu,Jin, Li,Yang, Binghui. 2018

[9]The life cycle of Hyalomma rufipes (Acari: Ixodidae) under laboratory conditions. Chen, Ze,Li, Youquan,Liu, Zhijie,Yang, Jifei,Yin, Hong. 2012

[10]Raising potential yield of short-duration rice cultivars is possible by increasing harvest index. Yin, Xiaohong,Jiang, Ligeng,Zou, Yingbin,Jiang, Ligeng,Zou, Yingbin,Deng, Guofu.

[11]Studying the Mechanism of Plasmopara viticola RxLR Effectors on Suppressing Plant Immunity. Li, Xinlong,Wu, Jiao,Zhang, Yali,Lu, Jiang,Yin, Ling. 2016

[12]Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels. Zhou, Chuanpeng,Ge, Xianping,Liu, Bo,Xie, Jun,Chen, Ruli,Zhou, Chuanpeng. 2014

[13]Effect of High Dietary Carbohydrate on Growth, Serum Physiological Response, and Hepatic Heat Shock Protein 70 Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures. Zhou, Chuanpeng,Liu, Bo,Xie, Jun,Ge, Xianping,Liu, Bo,Xie, Jun,Ge, Xianping. 2013

[14]Transcriptome analysis of the spleen of the darkbarbel catfish Pelteobagrus vachellii in response to Aeromonas hydrophila infection. Wen, Zhengyong,Yuan, Dengyue,Shao, Ting,Wang, Jun,Li, Huatao,Gong, Quan. 2017

[15]Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus). Yang, Chang-Geng,Sun, Bing,Liu, Shan-Shan,Chen, Song-Lin,Yang, Chang-Geng,Wang, Xian-Li,Zhang, Bo. 2013

[16]Identification and screening of effective protective antigens for channel catfish against Streptococcus iniae. Wang, Yajun,Wang, Erlong,He, Yang,Wang, Kaiyu,Yang, Qian,Wang, Jun,Geng, Yi,Ouyang, Ping,Lai, Weimin,Wang, Yajun,Wang, Kaiyu,Geng, Yi,Chen, Defang,Huang, Xiaoli. 2017

[17]Molecular characterization and expression analysis of a novel r-spondin member (rspo2l) in Chinese tongue sole (Cynoglossus semilaevis). Wei, Min,Zhao, Fa-zhen,Chen, Song-lin,Wei, Min,Xu, Wen-teng,Wang, Lei,Xiu, Yun-ji,Yang, Ying-ming,Li, Yang-zhen,Zhao, Fa-zhen,Chen, Song-lin,Wei, Min,Xu, Wen-teng,Wang, Lei,Xiu, Yun-ji,Yang, Ying-ming,Li, Yang-zhen,Zhao, Fa-zhen,Chen, Song-lin,Li, Hai-long. 2018

[18]Multiplex immune-related genes expression analysis response to bacterial challenge in mud crab, Scylla paramamosain. Zhang, Fengying,Jiang, Keji,Sun, Manman,Zhang, Dan,Ma, Lingbo. 2013

[19]The warm temperature acclimation protein Wap65 as an immune response gene: Its duplicates are differentially regulated by temperature and bacterial infections. Sha, Zhenxia,Yu, Peng,Takano, Tomokazu,Liu, Hong,Terhune, Jeffrey,Liu, Zhanjiang,Sha, Zhenxia,Liu, Hong. 2008

[20]Protochordate amphioxus is an emerging model organism for comparative immunology. Zhang, Shicui,Liang, Yujun,Ji, Guangdong,Zhuang, Zhimeng. 2009

作者其他论文 更多>>

微信小程序