Whole genome-wide transcript profiling to identify differentially expressed genes associated with seed field emergence in two soybean low phytate mutants
文献类型: 外文期刊
第一作者: Yuan, Fengjie
作者: Yuan, Fengjie;Yu, Xiaomin;Dong, Dekun;Yang, Qinghua;Fu, Xujun;Zhu, Shenlong;Zhu, Danhua
作者机构:
关键词: Low phytate seed;Soybean;Germination;Field emergence;Transcript
期刊名称:BMC PLANT BIOLOGY ( 影响因子:4.215; 五年影响因子:4.96 )
ISSN: 1471-2229
年卷期: 2017 年 17 卷
页码:
收录情况: SCI
摘要: Background: Seed germination is important to soybean (Glycine max) growth and development, ultimately affecting soybean yield. A lower seed field emergence has been the main hindrance for breeding soybeans low in phytate. Although this reduction could be overcome by additional breeding and selection, the mechanisms of seed germination in different low phytate mutants remain unknown. In this study, we performed a comparative transcript analysis of two low phytate soybean mutants (TW-1 and TW-1-M), which have the same mutation, a 2 bp deletion in GmMIPS1, but show a significant difference in seed field emergence, TW-1-M was higher than that of TW-1. Results: Numerous genes analyzed by RNA-Seq showed markedly different expression levels between TW-1-M and TW-1 mutants. Approximately 30,000-35,000 read-mapped genes and similar to 21000-25000 expressed genes were identified for each library. There were similar to 3900-9200 differentially expressed genes (DEGs) in each contrast library, the number of up-regulated genes was similar with down-regulated genes in the mutant TW-1and TW-1-M. Gene ontology functional categories of DEGs indicated that the ethylene-mediated signaling pathway, the abscisic acid-mediated signaling pathway, response to hormone, ethylene biosynthetic process, ethylene metabolic process, regulation of hormone levels, and oxidation-reduction process, regulation of flavonoid biosynthetic process and regulation of abscisic acid-activated signaling pathway had high correlations with seed germination. In total, 2457 DEGs involved in the above functional categories were identified. Twenty-two genes with 20 biological functions were the most highly up/down-regulated (absolute value Log2FC >5) in the high field emergence mutant TW-1-M and were related to metabolic or signaling pathways. Fifty-seven genes with 36 biological functions had the greatest expression abundance (FRPM >100) in germination-related pathways. Conclusions: Seed germination in the soybean low phytate mutants is a very complex process, which involves a series of physiological, morphological and transcriptional changes. Compared with TW-1, TW-1-M had a very different gene expression profile, which included genes related to plant hormones, antioxidation, anti-stress and energy metabolism processes. Our research provides a molecular basis for understanding germination mechanisms, and is also an important resource for the genetic analysis of germination in low phytate crops. Plant hormone-and antioxidation-related genes might strongly contribute to the high germination rate in the TW-1-M mutant.
分类号:
- 相关文献
作者其他论文 更多>>
-
Genetic and Phenotypic Characterization of Soybean Landraces Collected from the Zhejiang Province in China
作者:Yu, Xiaomin;Fu, Xujun;Yang, Qinghua;Jin, Hangxia;Zhu, Longming;Yuan, Fengjie;Yuan, Fengjie;Yuan, Fengjie;Yuan, Fengjie
关键词:soybean; landrace; germplasm resource; genetic diversity; phenotypic variation
-
Metabolic Rewiring in Tea Plants in Response to Gray Blight Disease Unveiled by Multi-Omics Analysis
作者:Zheng, Shiqin;Zheng, Shiqin;Du, Zhenghua;Wang, Xiaxia;Zheng, Chao;Yu, Xiaomin;Zheng, Shiqin;Wang, Zonghua;Wang, Zonghua
关键词:tea plant; gray blight disease; flavonoid; lignin; defense-related metabolites; metabolomics; transcriptomics
-
Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis
作者:Fan, Xiaocong;Chen, Shizhan;Wu, Wenjing;Sun, Guanghua;Yao, Shuaitao;Zhan, Weimin;Zhang, Yanpei;Wang, Lijian;Jiang, Liangliang;Yang, Jianping;Yang, Qinghua;Fan, Xiaocong;Chen, Shizhan;Wu, Wenjing;Sun, Guanghua;Yao, Shuaitao;Zhan, Weimin;Zhang, Yanpei;Wang, Lijian;Jiang, Liangliang;Yang, Jianping;Yang, Qinghua;Song, Meifang;Yan, Lei;Li, Hongdan;Zhang, Kang;Jiang, Liangliang
关键词:Zea mays L.; Cryptochrome; Photomorphogenesis; Shade avoidance syndrome; Hormone
-
Antioxidant Capacity of Free and Bound Phenolics from Olive Leaves: In Vitro and In Vivo Responses
作者:Li, Ting;Zhang, Jianming;Wu, Qinghang;Liu, Daqun;Zhang, Chengcheng;Li, Ting;Wu, Wenjun;Jiang, Chengying;Zhu, Shenlong;Niu, Erli;Wang, Shengfeng
关键词:olive leaves; free phenolics; bound phenolics; antioxidant properties; oxidative stress
-
Cryptochrome 1b represses gibberellin signaling to enhance lodging resistance in maize
作者:Chen, Shizhan;Fan, Xiaocong;Yao, Shuaitao;Liu, Tong;Ding, Wusi;Liu, Lei;Zhang, Menglan;Zhan, Weimin;Sun, Guanghua;Wang, Lijian;Jia, Xiaolin;Yang, Qinghua;Yang, Jianping;Chen, Shizhan;Fan, Xiaocong;Song, Meifang;Yao, Shuaitao;Liu, Tong;Ding, Wusi;Liu, Lei;Zhang, Menglan;Zhan, Weimin;Sun, Guanghua;Wang, Lijian;Jia, Xiaolin;Yang, Qinghua;Yang, Jianping;Song, Meifang;Liu, Lei;Li, Hongdan;Song, Meifang;Yan, Lei;Li, Hongdan;Yang, Jianping;Zhang, Kang
关键词:
-
Precise classification and regional delineation of maturity groups in soybean cultivars across China
作者:Song, Wenwen;Sun, Shi;Tian, Shiyan;Sun, Bincheng;Li, Wenbin;Liu, Lijun;Wang, Shuming;Xing, Han;Zhou, Xin'an;Nian, Hai;Lu, Wencheng;Han, Xiaozeng;Wang, Shouyi;Chen, Weiyuan;Guo, Tai;Song, Xiqing;Tian, Zhongyan;Cheng, Yanxi;Song, Shuhong;Fu, Lianshun;Wang, Huicai;Luo, Ruiping;Liu, Xueyi;Liu, Qi;Zhang, Guohong;Lu, Sihui;Xu, Ran;Li, Suzhen;Lu, Weiguo;Zhang, Qi;Wang, Zongbiao;Jiang, Chengong;Shen, Weiliang;Zhang, Mingrong;Zhu, Danhua;Wang, Ruizhen;Chen, Yuan;Wang, Tiejun;Zhu, Xingtao;Zhan, Yong;Gai, Junyi;Wu, Cunxiang;Han, Tianfu;Song, Wenwen;Liu, Luping;Sun, Shi;Wu, Tingting;Zeng, Haiyan;Tian, Shiyan;Jiang, Bingjun;Xu, Cailong;Yuan, Shan;Hou, Wensheng;Wu, Cunxiang;Han, Tianfu;Liu, Luping
关键词:Soybean; Maturity group; Precise classification; Optimum adaptation zone
-
Reticulate evolution of the tertiary relict Osmanthus
作者:Li, Yongfu;Li, Xuan;Zhang, Min;Yang, Qinghua;Duan, Yifan;Wang, Xianrong;Nie, Shuai;Nie, Shuai;Nie, Shuai;Xu, Wenbin
关键词:phylogenomics; introgression; reticulate evolution; historical biogeography