Insights from the Cold Transcriptome and Metabolome of Dendrobium officinale: Global Reprogramming of Metabolic and Gene Regulation Networks during Cold Acclimation

文献类型： 外文期刊

第一作者： Wu, Zhi-Gang

作者： Wu, Zhi-Gang;Jiang, Wu;Chen, Song-Lin;Tao, Zheng-Ming;Jiang, Cheng-Xi;Chen, Song-Lin;Mantri, Nitin

作者机构：

关键词： Cold acclimation;Dendrobium officinale;metabolic network;regulatory mechanism;signal transduction;transcriptome

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Plant cold acclimation (CA) is a genetically complex phenomenon involving gene regulation and expression. Little is known about the cascading pattern of gene regulatroy network and the link between genes and metabolites during CA. Dendrobium officinale (DOKM) is an important medicinal and ornamental plant and hypersensitive to low temperature. Here, we used the large scale metabolomic and transcriptomic technologies to reveal the response to CA in DOKM seedlings based on the physiological profile analyses. Lowering temperature from 4 to -2 degrees C resulted in significant increase (P < 0.01) in antioxidant activities and electrolyte leakage (EL) during 24 h. The fitness CA piont of 0 degrees C and control (20 degrees C) during 20 h were firstly obtained according to physiological analyses. Subsequently, massive transcriptome and metabolome reprogramming occurred during CA. The gene to metabolite network demonstrated that the CA associated processes are highly energy demanding through activating hydrolysis of sugars, amino acids catabolism and citrate cycle. The expression levels of 2,767 genes were significantly affected by CA, including 153-fold upregulation of CBF transcription factor, 56-fold upregulation of MAPKKK16 protein kinase. Moreover, the gene interaction and regulation network analysis revealed that the CA as an active process, was regulated at the transcriptional, post-transcriptional, translational and post-translational levels. Our findings highligted a comprehensive regulatory mechanism including cold signal transduction, transcriptional regulation, and gene expression, which contributes a deeper understanding of the highly complex regulatory program during CA in DOKM. Some marker genes identified in DOKM seedlings will allow us to understand the role of each individual during CA by further functional analyses.

分类号：

  • 相关文献

[1]De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments. Li, Jin,Liu, Hailiang,Xia, Wenwen,Mu, Jianqiang,Feng, Yujie,Liu, Ruina,Wang, Aiying,Lin, Zhongping,Zhu, Jianbo,Chen, Xianfeng,Liu, Hailiang,Yan, Panyao,Chen, Xianfeng,Lin, Zhongping,Guo, Yong. 2017

[2]Differential gene expression and associated QTL mapping for cotton yield based on a cDNA-AFLP transcriptome map in an immortalized F-2. Liu, Renzhong,Wang, Baohua,Guo, Wangzhen,Zhang, Tianzhen,Liu, Renzhong,Wang, Liguo.

[3]Transcriptome analysis reveals that distinct metabolic pathways operate in salt-tolerant and salt-sensitive upland cotton varieties subjected to salinity stress. Guo, Jinyan,Zhang, Liwei,Xu, Wenying,Su, Zhen,Shi, Gongyao,Guo, Xiaoyan,Hua, Jinping,Wang, Yumei.

[4]Reconstruction of metabolic network in the bovine mammary gland tissue. Wang, Changfa,Wang, Ji,Ju, Zhihua,Zhai, Ruiyan,Zhou, Lei,Li, Qiuling,Li, Jianbin,Li, Rongling,Huang, Jinming,Zhong, Jifeng.

[5]ocsESTdb: a database of oil crop seed EST sequences for comparative analysis and investigation of a global metabolic network and oil accumulation metabolism. Ke, Tao,Yu, Jingyin,Dong, Caihua,Mao, Han,Hua, Wei,Liu, Shengyi,Ke, Tao. 2015

[6]Determination of Pyrethroids in Dendrobium officinale by Ultrasound/Microwave-Assisted Solid-Liquid-Solid Dispersive Extraction, Gas Chromatography, and Triple-Quadrupole Mass Spectrometry. Wu, Huizhen,Li, Zuguang,Qian, Mingrong,Wang, Jianmei,Zhang, Hu.

[7]Ultrasound/microwave-assisted solid-liquid-solid dispersive extraction with high-performance liquid chromatography coupled to tandem mass spectrometry for the determination of neonicotinoid insecticides in Dendrobium officinale. Zheng, Shuilian,Wu, Huizhen,Li, Zuguang,Wang, Jianmei,Zhang, Hu,Qian, Mingrong.

[8]Construction of a genome-scale metabolic network of the plant pathogen Pectobacterium carotovorum provides new strategies for bactericide discovery. Wang, Cheng,Chang, Ji-Wei,Kong, De-Xin,Chen, Ling-Ling,Wang, Cheng,Deng, Zhi-Luo,Xie, Zhi-Ming,Chu, Xin-Yi,Chang, Ji-Wei,Kong, De-Xin,Zhang, Hong-Yu,Chen, Ling-Ling,Li, Bao-Ju.

[9]Hydrogen sulphide alleviates oxidative damage and enhances light energy transformation under high light for Dendrobium officinale. Fan, Honghong,Guan, Lei,Li, Tingchun,Wu, Qiuju,Wu, Meijuan,Cai, Yongping,Lin, Yi,Li, Tingchun.

[10]Transcript characteristic of myostatin in sheep fibroblasts. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Zhang, Xiaoning,Zhao, Fuping,Zhou, Xinlei,Zhang, Li,Wei, Caihong,Du, Lixin,Ren, Hangxing,Sheng, Xihui,Li, Shangang.

[11]Drought-induced site-specific DNA methylation and its association with drought tolerance in rice (Oryza sativa L.). Wang, Wen-Sheng,Pan, Ya-Jiao,Zhao, Xiu-Qin,Zhu, Ling-Hua,Fu, Bin-Ying,Li, Zhi-Kang,Wang, Wen-Sheng,Dwivedi, D.,Ali, J.,Li, Zhi-Kang.

[12]Identification of differentially expressed genes and pathways for intramuscular fat metabolism between breast and thigh tissues of chickens. Cui, Huanxian,Zheng, Maiqing,Zhao, Guiping,Liu, Ranran,Wen, Jie,Cui, Huanxian,Zheng, Maiqing,Zhao, Guiping,Liu, Ranran,Wen, Jie. 2018

[13]Research progress in allele-specific expression and its regulatory mechanisms. Gaur, Uma,Mei, Shuqi,Liu, Guisheng,Gaur, Uma,Mei, Shuqi,Liu, Guisheng,Li, Kui.

[14]Isolation and characterization of the carotenoid biosynthetic genes LCYB, LCYE and CHXB from strawberry and their relation to carotenoid accumulation. Zhu, Haisheng,Chen, Mindong,Wen, Qingfang,Li, Yongping,Zhu, Haisheng,Chen, Mindong,Wen, Qingfang,Li, Yongping.

[15]Cloning of the strawberry xanthophyll cycle genes VDE and ZEP and characterization of their responses to high light stress. Zhu, Haisheng,Chen, Mindong,Bin, Wang,Wen, Qingfang.

[16]Expression and regulation of a cold-responsive gene, CsCBF in Citrus sinensis (L.) Osbeck under low temperature, high salinity and abscisic acid. He, L. G.,Jiang, Y. C.,Wang, H. L.,Xu, M.,Sun, Z. H.. 2016

[17]Differentially expressed genes under cold acclimation in Physcomitrella patens. Sun, Ming-Ming,Li, Lin-Hui,Xie, Hua,Ma, Rong-Cai,He, Yi-Kun. 2007

[18]Global transcriptome profiles of Camellia sinensis during cold acclimation. Wang, Xin-Chao,Ma, Chun-Lei,Cao, Hong-Li,Yue, Chuan,Hao, Xin-Yuan,Chen, Liang,Ma, Jian-Qiang,Jin, Ji-Qiang,Yang, Ya-Jun,Wang, Xin-Chao,Ma, Chun-Lei,Cao, Hong-Li,Yue, Chuan,Hao, Xin-Yuan,Chen, Liang,Ma, Jian-Qiang,Jin, Ji-Qiang,Yang, Ya-Jun,Zhao, Qiong-Yi,Yue, Chuan,Li, Xuan,Zhao, Qiong-Yi,Zhang, Zong-Hong. 2013

[19]Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season. Yue, Chuan,Cao, Hong-Li,Wang, Lu,Zhou, Yan-Hua,Huang, Yu-Ting,Hao, Xin-Yuan,Wang, Yu-Chun,Wang, Bo,Yang, Ya-Jun,Wang, Xin-Chao,Yue, Chuan,Cao, Hong-Li,Wang, Lu,Zhou, Yan-Hua,Huang, Yu-Ting,Hao, Xin-Yuan,Wang, Yu-Chun,Wang, Bo,Yang, Ya-Jun,Wang, Xin-Chao,Wang, Lu,Hao, Xin-Yuan,Wang, Yu-Chun,Wang, Bo,Yang, Ya-Jun,Wang, Xin-Chao.

[20]Autumn dormancy regulates the expression of cas18, vsp and corF genes during cold acclimation of lucerne (Medicago sativa L.). Liu, Zhi-ying,Liu, Zhi-ying,Li, Xi-liang,Yan, Ya-fei,Gao, Run,Sun, Qi-zhong,Wang, Zong-li,Yang, Guo-feng,Sun, Juan,Wang, Zong-li.

作者其他论文 更多>>

微信小程序