Overexpression of StERECTA enhances drought tolerance in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Liu, Xuan

作者： Liu, Xuan;Yang, Wenjing;Zhang, Li;Nie, Fengjie;Liu, Xuan;Yang, Wenjing;Zhang, Li;Nie, Fengjie;Gong, Lei;Zhang, Hongxia;Zhang, Hongxia

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关键词： ERECTA; Potato; Drought stress; Gene expression; ABA; Stomatal morphology

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：4.1； 五年影响因子：4.3 ）

ISSN： 0176-1617

年卷期： 2024 年 303 卷

页码：

收录情况： SCI

摘要： Drought is a major abiotic stresses that severely hinder plant growth and agricultural productivity. The receptorlike kinase gene, ERECTA, has been proved to play important role in promoting the response to abiotic stress in crops. However, the specific molecular mechanisms underlying the drought resistance mediated by ERECTA in potato (Solanum tuberosum L.) are not well understood. In this study, sequence analysis confirmed that the StERECTA gene contains eight leucine-rich repeat (LRR) domains and an S_TKc domain, and these domains were highly conserved in Solanaceae family. Under drought stress, Arabidopsis thaliana strains overexpressing StERECTA showed increased biomass, proline (PRO) content, and antioxidant enzyme activities compared to the wildtype strains while the mutant ERECTA strain (er105) exhibited opposite phenotype. Additionally, StERECTA overexpression upregulated the expression of drought response marker genes (LEA3, DREB2A and P5CS1), improved levels of ABA and auxin, reduced stomatal density and relative expression level of stomatal development related genes (SPCH, FAMA and MUTE). Furthermore, Co-immunoprecipitation (Co-IP) assays demonstrated that StERECTA physically interacted with the YODA protein. In conclusion, our study provides new insights into the role and regulatory mechanism of StERECTA in response to drought stress. These findings may serve as a basis for genetic improvement of potato to enhance their tolerance to abiotic stress.

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