Proteomic Analysis of Fruit Bending in Cucumber (Cucumis sativus L.)

文献类型： 外文期刊

第一作者： Wang Li-li

作者： Wang Li-li;Qin Zhi-wei;Zhou Xiu-yan;Zhang Peng

作者机构：

关键词： cucumber;fruit;bending;proteome

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2014 年 13 卷 5 期

页码：

收录情况： SCI

摘要： In cucumber, fruit shape is an important quality criterion, and fruit bending is known to limit growth, yield, and taste. To investigate the post-transcriptional changes that regulate fruit bending and to better understand the underlying molecular mechanisms, we generated a proteomic profile of the abdomen and back of cucumber bending fruit. Two-dimensional gel electrophoresis (2-DE) allowed the detection of approximately 900 distinct protein spots in each gel, 32 of which were differentially expressed in the abdomen and back of bending cucumber fruit. Ten of the differentially expressed proteins were analyzed using matrix-assisted laser ionization time of flight mass spectrometry (MALDI-TOF/MS). A search of primary databases showed that the identified proteins are involved in various metabolic processes and cellular responses, including photosynthesis metabolism, energy metabolism, defense and stress response, and regulation. The identified proteins included large subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase,which are involved in photosynthesis and photorespiratory metabolism, and isocitrate dehydrogenase, which is involved in the tricarboxylic acid cycle. It is possible that imbalances in catabolic and anabolic processes directly affect the bending of cucumber fruit. The predicted function of the cobalamin-independent methionine synthase isozyme is closely related to ethylene biosynthesis; fruit bending may be regulated by ethylene, or by ethylene signaling crosstalk during fruit development. The 14-3-3 protein is usually considered to be a regulation-related protein, which plays a role in regulating cell hyperplasia, cell differentiation during growth, and apoptosis during senescence. Involvement of guanosine triphosphate (GTP)-binding proteins in signal transmission is known to regulate the development of cells in cucumber fruits and to play a role in fruit shape variation. Patterns of protein expression showed high repeatability. We hypothesize that these proteins may play an important role in growth and bending of cucumber fruits. The results of our study provide insight into the genetic mechanism underlying fruit bending in cucumber, and may help to promote cultivation of new varieties with superior fruits.

分类号：

  • 相关文献

[1]Characterization and expression profiling of cucumber kinesin genes during early fruit development: revealing the roles of kinesins in exponential cell production and enlargement in cucumber fruit. Yang, Xue Yong,Wang, Yan,Jiang, Wei Jie,Liu, Xiao Ling,Zhang, Xiao Meng,Yu, Hong Jun,Huang, San Wen,Liu, Guo Qin. 2013

[2]Apple MdACS6 Regulates Ethylene Biosynthesis During Fruit Development Involving Ethylene-Responsive Factor. Li, Tong,Tan, Dongmei,Jiang, Zhongyu,Wei, Yun,Zhang, Lichao,Li, Xinyue,Yuan, Hui,Wang, Aide,Liu, Zhi.

[3]Fruit protected cultivation in China. Gao, H,Wang, S,Wang, J.

[4]Determining hydrogen cyanamide in fruit by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate and HPLC with fluorescence detection. Cai, Xiao-Ming,Zhao, Hua,Wu, Min,Hu, Xiu-Qing,He, Hong-Mei,Zhang, Chang-Peng,Zhang, Chun-Rong,Li, Zhen,Cai, Xiao-Ming,Zhao, Hua,Wu, Min,Hu, Xiu-Qing,He, Hong-Mei,Zhang, Chang-Peng,Zhang, Chun-Rong,Li, Zhen,Cai, Xiao-Ming,Zhao, Hua,Wu, Min,Hu, Xiu-Qing,He, Hong-Mei,Zhang, Chang-Peng,Zhang, Chun-Rong,Li, Zhen.

[5]Rapid Analysis of Tetraconazole Residues in Fruits and Vegetables using Ethyl Acetate Extraction and Gas Chromatography-tandem Mass Spectrometry. Doug, Fengshou,Liu, Xingang,Li, Jing,Li, Yuanbo,Zheng, Yongquan,Shan, Weili.

[6]GC-MS Analysis of Volatile Components in Chinese Wild Strawberry (F. nilgerrensis Schlecht.). Zhao, M. Z.,Wang, J.,Wang, Z. W.,Qian, Y. M.,Wu, W. M..

[7]Evaluation of nine pesticide residues in three minor tropical fruits from southern China. Yang, Xinfeng,Luo, Jinhui,Li, Shuhuai,Liu, Chuanhua,Yang, Xinfeng,Luo, Jinhui,Li, Shuhuai,Liu, Chuanhua,Yang, Xinfeng,Luo, Jinhui,Li, Shuhuai,Liu, Chuanhua.

[8]Meta-analysis of food processing on pesticide residues in fruits. Liang, Y.,Liu, Y.,Liu, X. J.,Liang, Y.,Ding, Y.,Liu, X. J..

[9]Volatile Characterization of Major Apricot Cultivars of Southern Xinjiang Region of China. Feng, Jian-rong,Liu, Hai-nan,Liu, Xiao-fang,Lu, Xiao-yan,Xi, Wan-peng,Li, Wen-hui,Li, Wen-hui.

[10]Genotypic differences in pod wall and seed growth relate to invertase activities and assimilate transport pathways in asparagus bean. Liu, Yong-Hua,Li, Guo-Jing,Wu, Xiao-Hua,Wang, Bao-Gen,Xu, Pei,Hu, Ting-Ting,Lu, Zhong-Fu,Liu, Yong-Hua,Cao, Jia-Shu,Patrick, John W.,Ruan, Yong-Ling.

[11]Occurrence of patulin in various fruit products and dietary exposure assessment for consumers in China. Ji, Xiaofeng,Li, Rui,Yang, Hua,Qi, Peipei,Xiao, Yingping,Qian, Mingrong,Ji, Xiaofeng,Li, Rui,Yang, Hua,Qi, Peipei,Xiao, Yingping,Qian, Mingrong,Ji, Xiaofeng,Li, Rui,Yang, Hua,Qi, Peipei,Xiao, Yingping,Qian, Mingrong.

[12]Differentially expressed cDNAs at the early stage of banana ripening identified by suppression subtractive hybridization and cDNA microarray. Xu, Bi Yu,Su, Wei,Liu, Ju Hua,Wang, Jia Bao,Jin, Zhi Qiang.

[13]A Study on Anatomical Structure of Leaves and Fruits in Ziziphus jujuba 'Jingzao 39'. Pan, Q. H.,Zhang, Y. P.,Li, H. L..

[14]Effects of postharvest methyl jasmonate treatment on aromatic volatile biosynthesis by 'Nanguoli' fruit at different harvest maturity stages. Qin, G. H.,Wei, S. W.,Tao, S. T.,Zhang, H. P.,Huang, W. J.,Yao, G. F.,Zhang, S. L.,Qin, G. H.,Wei, S. W.,Sha, S. F..

[15]Simultaneous Determination of Plant Growth Regulators in Fruit by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Modified QuEChERS Procedure. Yan Zhen,Nie Ji-Yun,Cheng Yang,Li Zhi-Xia,Xu Guo-Feng,Li Hai-Fei. 2017

[16]Variations in contents of (-)-epicatechin and activities of phenylalanine ammonialyase and polyphenol oxidase of longan fruit during development. Yu, Chunyan,Sun, Jian,Yang, Bao,Jiang, Yueming,Xiang, Xu. 2010

[17]Effects of Citric Acid Treatments on the Postharvest Fruit Quality of Ziziphus jujuba Mill. 'Linyilizao'. Zhao, Z. H.,Liu, M. J.,Liu, P.,Liu, X. Y.,Dai, L.,Yang, L.,Tian, S. L.. 2009

[18]Determination of Semiamitraz Residue in Fruits by Solid Phase Extraction-High Performance Liquid Chromatography. Feng Xiao-Yuan,Yang Yuan,Shi Lei,Zhang Kai-Chun,Wang Dong,Zhang Jia,Feng Xiao-Yuan,Wang Bao-Gang. 2010

[19]Measurement of the Fluctuation Risk of the China Fruit Market Price based on VaR. Wang Chuan,Zhao Junye,Huang Min,Wang Chuan,Zhao Junye,Huang Min. 2010

[20]Predicting oleocellosis sensitivity in citrus using VNIR reflectance spectroscopy. Zheng, Yongqiang,He, Shaolan,Yi, Shilai,Deng, Lie,Zheng, Yongqiang,Zhou, Zhiqin,Mao, Shasha,Zhao, Xuyang. 2010

作者其他论文 更多>>

微信小程序