Establishment of a highly efficient regeneration method from the scraped broken embryo of mature barley seed
文献类型: 外文期刊
第一作者: Hua, Wei
作者: Hua, Wei;Zhu, Jinghuan;Shang, Yi;Wang, Junmei;Jia, Qiaojun;Lin, Feng;Yang, Jianming
作者机构:
关键词: Barley;callus induction;mature embryo;plant regeneration;scraped broken embryo
期刊名称:CANADIAN JOURNAL OF PLANT SCIENCE ( 影响因子:1.018; 五年影响因子:1.242 )
ISSN: 0008-4220
年卷期: 2013 年 93 卷 6 期
页码:
收录情况: SCI
摘要: An efficient and reproducible plant regeneration system for mature barley would represent a significant innovation for barley tissue culture and genetic transformations to optimize barley crop traits. To address this need, we used scraped broken embryos (SBEs) from dried barley seeds to establish a highly efficient regeneration system. Mature SBEs cultured in an induction medium with 2 mg L-1 2,4-D offered the best callus formation (92.99% induction efficiency) and no germination. Furthermore, call arising from SBEs had the highest regeneration frequency (69.72%) in FHG medium with 1 mg L-1 6-benzylaminopurine. We then compared three different cultivars: Golden Promise, Zaoshu3, and Zhepi8 for callogenesis and regeneration using an established protocol. Zhepi8 had the greatest callus induction (92.78%) and regeneration (68.24%). Our innovative methods for mature barley plant reproduction will contribute to improvements in genetic barley transformation as an alternate regeneration system.
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