Construction and analysis of a subtractive cDNA library of early embryonic development in duck

文献类型： 外文期刊

第一作者： Liu, Y. L.

作者： Liu, Y. L.;Zhong, L. X.;Shi, F. X.;Liu, Y. L.;Zhong, L. X.;Li, J. J.;Shen, J. D.;Wang, D. Q.;Tao, Z. R.;Lu, L. Z.

作者机构：

关键词： Duck;Embryo development;Subtractive hybridization;Duplex-specific nuclease;Nanog

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2013 年 12 卷 3 期

页码：

收录情况： SCI

摘要： Several studies have documented the process of early embryonic development in poultry; however, the molecular mechanisms underlying its developmental regulation are poorly understood, particularly in ducks. In this study, we analyzed differential gene expression of embryos 6 and 25 h following oviposition to determine which genes regulate the early developmental stage in ducks. Among 216 randomly selected clones, 39 protein-encoding cDNAs that function in metabolism, transcription, transportation, proliferation/apoptosis, cell cycle, cell adhesion, and methylation were identified. Additionally, the full-length cDNA of the Nanog gene, encoding a 302-amino acid protein, was obtained. Quantitative real-time polymerase chain reaction analyses were performed to detect expression levels of the selected genes during early and late embryonic stages, which revealed that these genes are expressed in a particular spatial and temporal pattern. These results indicate that these genes may play pivotal roles in the process of area pellucida formation through a complex and precise regulatory network during development in duck embryos.

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