Genome-wide identification, characterization, and expression analysis of the MLO gene family in Cucumis sativus

文献类型： 外文期刊

第一作者： Zhou, S. J.

作者： Zhou, S. J.;Jing, Z.;Shi, J. L.

作者机构：

关键词： MLO;Cucumber;Bioinformatics;Powdery mildew

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2013 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Mildew resistance locus o (MLO) is a plant-specific seven-transmembrane (TM) gene family. Several studies have revealed that certain members of the MLO gene family mediate powdery mildew susceptibility in three plant species, namely, Arabidopsis, barley, and tomato. The sequenced cucumber genome provides an opportunity to conduct a comprehensive overview of the MLO gene family. Fourteen genes (designated CsMLO01 through CsMLO14) have been identified within the Cucumis sativus genome by using an in silico cloning method with the MLO amino acid sequences of Arabidopsis thaliana and rice as probes. Sequence alignment revealed that numerous features of the gene family, such as TMs, a calmodulin-binding domain, peptide domains I and II, and 30 important amino acid residues for MLO function, are well conserved. Phylogenetic analysis of the MLO genes from cucumber and other plant species reveals seven different clades (I through VII). Three of these clades comprised MLO genes from A. thaliana, rice, maize, and cucumber, suggesting that these genes may have evolved after the divergence of monocots and dicots. In silico mapping showed that these CsMLOs were located on chromosomes 1, 2, 3, 4, 5, and 6 without any obvious clustering, except CsMLO01. To our knowledge, this paper is the first comprehensive report on MLO genes in C. sativus. These findings will facilitate the functional characterization of the MLOs related to powdery mildew susceptibility and assist in the development of disease resistance in cucumber.

分类号：

  • 相关文献

[1]Genomic identification, phylogeny, and expression analysis of MLO genes involved in susceptibility to powdery mildew in Fragaria vesca. Miao, L. X.,Zhang, Y. C.,Yang, X. F.,Zhang, H. Q.,Zhang, Z. F.,Wang, Y. Z.,Jiang, G. H.,Jiang, M.. 2016

[2]Identification of candidate MLO powdery mildew susceptibility genes in cultivated Solanaceae and functional characterization of tobacco NtMLO1. Appiano, Michela,Visser, Richard G. F.,Bai, Yuling,Pavan, Stefano,Bracuto, Valentina,Ricciardi, Luigi,Catalano, Domenico,Zheng, Zheng,Lotti, Concetta.

[3]Monocot and dicot MLO powdery mildew susceptibility factors are functionally conserved in spite of the evolution of class-specific molecular features. Appiano, Michela,Martinez, Miguel Santillan,Visser, Richard G. F.,Bai, Yuling,Catalano, Domenico,Lotti, Concetta,Zheng, Zheng,Ricciardi, Luigi,Pavan, Stefano. 2015

[4]Genome-wide identification and analysis of heat shock transcription factor family in cucumber (Cucumis sativus L.). Zhou, Shengjun,Zhang, Peng,Jing, Zange,Shi, Jianlei. 2013

[5]Genome-wide characterization and comparative analysis of the MLO gene family in cotton. Xiaoyan Wang,Qifeng Ma,Lingling Dou,Zhen Liu,Renhai Peng,Shuxun Yu. 2016

[6]Isolation, characterization, and expression analysis of CmMLO2 in muskmelon. Cheng, Hong,Kong, Weiping,Hou, Dong,Tao, Xinglin,Lv, Junfeng.

[7]Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat. Li, AL,Kong, XY,Zhou, RH,Ma, ZY,Jia, JZ.

[8]Molecular cloning and expression analysis of CmMlo1 in melon. Cheng, Hong,Kun, Weiping,Liu, Dongshun,Su, Yongquan,He, Qiwei.

[9]Molecular cloning and characterization of a Mlo gene in rubber tree (Hevea brasiliensis). Qin, Bi,Zheng, Fucong,Zhang, Yu.

[10]Exploring laccase genes from plant pathogen genomes: a bioinformatic approach. Peng, B. Z.,Li, P. Q.,Fu, L.,Yu, X. M.. 2015

[11]Sequence analysis of a putative goose RIG-I gene. Li, Guoqin,Li, Jinjun,Tian, Yong,Wang, Deqian,Shen, Junda,Tao, Zhengrong,Xu, Jian,Lu, Lizhi. 2012

[12]High-throughput sequencing of highbush blueberry transcriptome and analysis of basic helix-loop-helix transcription factors. Song Yang,Liu Hong-di,Zhang Hong-jun,Wang Hai-bo,Liu Feng-zhi,Zhou Qiang,Zhang Zhi-dong,Li Ya-dong. 2017

[13]Identification of a novel S-RNase gene and S-genotypes of four pear (Pyrus pyrifolia) cultivars. Zhang Lin,Hu Jiao,Tan Xiaofeng,Long Hongxu,Yuan Deyi,Li Xiugen. 2010

[14]Promoter Characterization and Expression Pattern Analysis of Porcine TCAP Gene. Qiao, Mu,Wu, Huayu,Huang, Jingshu,Peng, Xianwen,Liu, Guisheng,Feng, Zheng,Mei, Shuqi. 2012

[15]Identification, characterization, and expression of a novel P450 gene encoding CYP6AE25 from the Asian corn borer, Ostrinia furnacalis. Wang, Jian-hua,Liu, Zhi-xin,Kulye, Mahesh,Zeng, Hong-mei,Yang, Feng-shan,Zhang, Yi-tong,Xiao, Luo. 2011

[16]Progress in expressed sequence tags (EST) project of plant genome. Luo, M,Jia, JZ. 2001

[17]Structure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus. Jin Li,Wang HongYan,Wang XiaoNing,Yang Zhong,Zhong Yang,Zhao GuoPing,Wu Di,Xu TianLei,Sun Jing,Cao ZhiWei,Wu Di,Yang Zhong,Zhong Yang,Li YiXue,Cao ZhiWei,Dai JianXin,Wang Hao,Guo YaJun,Wang XiaoNing,Dai JianXin,Wang Hao,Guo YaJun,Che XiaoYan,Huang Zhong,Lan Ke,Sun Bing,Wu Fan,Yuan ZhenAn,Zhang Xi,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Ding GuoHui,He YunGang,Bian Chao,Jin Li,Zhao GuoPing,Li YiXue,Zhou ZhengFeng,Xiong Hui,Dong Hui,Jin WeiRong,Jin Li,Zhao GuoPing,Zhou XiaoNong,Zhou JiaHai,Ma ZhiYong,Tong GuangZhi,Zhao GuoPing. 2009

[18]Comparative Genomics of Mycoplasma bovis Strains Reveals That Decreased Virulence with Increasing Passages Might Correlate with Potential Virulence-Related Factors. Rasheed, Muhammad A.,Qi, Jingjing,Zhu, Xifang,He Chenfei,Menghwar, Harish,Khan, Farhan A.,Gang Zhao,Zubair, Muhammad,Chen, Huanchun,Guo, Aizhen,Rasheed, Muhammad A.,Zhu, Xifang,He Chenfei,Menghwar, Harish,Khan, Farhan A.,Gang Zhao,Zubair, Muhammad,Hu, Changmin,Chen, Yingyu,Chen, Huanchun,Guo, Aizhen,Rasheed, Muhammad A.,Qi, Jingjing,Chen, Huanchun,Guo, Aizhen,Chen, Huanchun,Guo, Aizhen. 2017

[19]Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG(1) from peanut (Arachis hypogaea L.). Wan, Shu-bo,Shan, Shi-hua,Zhang, Ting-ting,Li, Chun-juan,Yang, Chen,Yan, Cai-xia,Yang, Chen. 2011

[20]Ecogenomic survey of plant viruses infecting Tobacco by Next generation sequencing. Akinyemi, Ibukun A.,Qi, Shuishui,Wu, Qingfa,Wang, Fang,Zhou, Benguo. 2016

作者其他论文 更多>>

微信小程序