Halovirs I-K, antibacterial and cytotoxic lipopeptaibols from the plant pathogenic fungus Paramyrothecium roridum NRRL 2183

文献类型： 外文期刊

第一作者： Xiao, Dongliang

作者： Xiao, Dongliang;Li, Tianyi;Li, Wenhua;Zhang, Liwen;Yue, Qun;Xu, Yuquan;Wang, Chen;Zhang, Mei;Chen, Xinqi;Wu, Ping;Wei, Xiaoyi

作者机构：

期刊名称：JOURNAL OF ANTIBIOTICS （ 影响因子：3.424； 五年影响因子：3.066 ）

ISSN： 0021-8820

年卷期： 2022 年 75 卷 5 期

页码：

收录情况： SCI

摘要： Three new lipopeptaibols, halovirs I-K (1-3), were isolated from the solid culture of the phytopathogenic fungus Paramyrothecium roridum NRRL 2183. Their planar structures, which consist of a hexapeptide backbone and acyl substitutions at the N- and C-termini, were elucidated by comprehensive analysis of the 1D and 2D NMR spectroscopic data along with the detailed interpretation of the MS/MS fragmentation pattern. Absolute configurations of the amino acid/1,2-amino alcohol residues were determined using the advanced Marfey's method. Bioinformatics analysis of the genome assembly of P. roridum NRRL 2183 revealed a gene cluster that is likely responsible for the biosynthesis of halovirs I-K. Analysis of the module and domain organization of the putative halovir synthetase PrHalA indicated that the assembly of 1-3 proceeds in an unconventional nonlinear fashion. 1 and 2 exhibited potent antibacterial activity against both antibiotic-sensitive and multidrug-resistant Gram-positive pathogens. These lipopeptaibols also displayed significant cytotoxicity toward human lung carcinoma A549, human breast carcinoma MCF-7, and human cervical carcinoma HeLa cells with IC50 values ranging from 1.3 to 3.3 mu M.

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