Sequence analysis of a putative goose RIG-I gene

文献类型: 外文期刊

第一作者: Li, Guoqin

作者: Li, Guoqin;Li, Jinjun;Tian, Yong;Wang, Deqian;Shen, Junda;Tao, Zhengrong;Xu, Jian;Lu, Lizhi

作者机构:

关键词: Goose;RIG-I;cDNA;mRNA expression;bioinformatics

期刊名称:CANADIAN JOURNAL OF ANIMAL SCIENCE ( 影响因子:1.015; 五年影响因子:1.094 )

ISSN: 0008-3984

年卷期: 2012 年 92 卷 2 期

页码:

收录情况: SCI

摘要: Li, G., Li, J., Tian, Y., Wang, DE., Shen, J., Tao, Z., Xu, J. and Lu, L. 2012. Sequence analysis of a putative goose RIG-I gene. Can. J. Anim. Sci. 92: 143-151. Retinoid acid-inducible gene-I (RIG-I) is a critical cytoplasmic RNA sensor which plays an important role in the recognition of, and response to, influenza virus and other RNA viruses. In the present study, A 3808-bp cDNA encoding goose RIG-I (goRIG-I) was cloned from splenic lymphocytes of geese using RT-PCR and rapid amplification of cDNA ends (RACE) techniques. The encoded protein, which is predicted to consist of 933 amino acids, has a molecular weight of 106.4 kDa and includes an N-terminal caspase recruitment domain (CARD), a domain with the signature of DExD/H box helicase (helicase domain), and a C-terminal repression domain (RD) similar to duck RIG-I (duRIG-I), human RIG-I, and mouse RIG-I. The goRIG-I showed 93.8 and 78.0% amino acid sequence identity with previously described duRIG-I and finch RIG-I, respectively, and 48.9-53.0% sequence identity with mammalian homologs. Quantitative RT-PCR analysis indicated that the goRIG-I gene is strongly expressed in the liver, lung, brain, spleen, and bursa of Fabricius. These findings lay the foundation for further research on the function and mechanism of avian RIG-I in innate immunity.

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