Development of a one-step real-time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu virus

文献类型: 外文期刊

第一作者: Yun, Tao

作者: Yun, Tao;Ni, Zheng;Hua, Jionggang;Ye, Weicheng;Chen, Liu;Zhang, Shuai;Zhang, Yan;Zhang, Cun;Zhang, Shuai

作者机构:

关键词: Duck Tembusu virus;Real-time RT-PCR;Minor-groove-binding probe;Diagnostic assay

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN: 0166-0934

年卷期: 2012 年 181 卷 2 期

页码:

收录情况: SCI

摘要: The design and development of a 3'-conjugated minor-groove-binding (MGB) probe for a real-time RT-PCR assay allowing for the rapid, sensitive, and specific detection of duck Tembusu virus (DTMUV) RNA are described. This assay targeted the 3' terminal non-coding region (NCR) of the TMUV genome and detected 1 x 10(1) copies of RNA per reaction without cross-reaction with other duck pathogens. The linear range of detection was 2 x 10(1)-2 x 10(8) copies/mu l. The assay was rapid, requiring just over 2.0 h, including the nucleic acid extraction step. Therefore, this assay is an excellent tool for research routine diagnostic applications, and study of the epidemiology of TMUV infections among duck flocks. (C) 2012 Elsevier B.V. All rights reserved.

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