OmpA is involved in the invasion of duck brain microvascular endothelial cells by Riemerella anatipestifer

文献类型： 外文期刊

第一作者： Zou, Ronghua

作者： Zou, Ronghua;Wu, Xiaoni;Chen, Qiwei;Gong, Xiaowei;Chu, Yuefeng;Zheng, Fuying

作者机构：

关键词： Riemerella anatipestifer; Duck brain microvascular endothelial cells; Blood brain barrier; OmpA protein

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 0378-1135

年卷期： 2023 年 280 卷

页码：

收录情况： SCI

摘要： Bacterial meningitis is a major cause of morbidity and mortality. Despite advances in antimicrobial chemo-therapy, the disease remains detrimental to humans, livestock, and poultry. Riemerella anatipestifer is a gram -negative bacterium causing duckling serositis and meningitis. However, the virulence factors contributing to its binding and invasion of duck brain microvascular endothelial cells (DBMECs) and penetration of the blood-brain barrier (BBB) have never been reported. In this study, immortalized DBMECs were successfully generated and used as an in vitro-model of duck BBB. Furthermore, ompA gene deletion mutant of the pathogen and multiple complemented strains carrying the complete ompA gene and its truncated forms were constructed. Bacterial growth, invasion, and adhesion assays and animal experiments were performed. The results show that the OmpA protein of R. anatipestifer had no effect on bacterial growth and adhesion ability to DBMECs. The role of OmpA in the invasion of R. anatipestifer into DBMECs and duckling BBB was confirmed. The amino acids 230-242 of OmpA represents a key domain involved in R. anatipestifer invasion. In addition, another OmpA1164 protein constituted by the amino acids 102-488 within OmpA could function as a complete OmpA. The signal peptide sequence from amino acids 1-21 had no significant effect on OmpA functions. In conclusion, this study illustrated that OmpA is an important virulence factor mediating R. anatipestifer invasion of DBMECs and penetration of the duckling BBB.

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