Expression of the B Subunit of Escherichia coli Heat-Labile Enterotoxin in Transformed Bombyx mori BmN Cells

文献类型： 外文期刊

第一作者： Zhou, Wen-Lin

作者： Zhou, Wen-Lin;Cao, Jin-Ru;Ye, Ai-Hong;Weng, Hong-Biao;He, Li-Hua;Wang, Yong-Qiang;Gong, Cheng-Liang;Xue, Ren-Yu;Cao, Guang-Li;Gong, Cheng-Liang;Xue, Ren-Yu;Cao, Guang-Li

作者机构：

关键词： Escherichia coli heat-labile enterotoxin;piggyBac transposon;Bombyx mori;BmN cell;genetic transformation

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 20 期

页码：

收录情况： SCI

摘要： The non-toxic B subunit of Escherichia coli heat-labile enterotoxin (LTB) is a potent mucosal immunogen and immunoadjuvant for coadministered antigens. To obtain transformed silkworm cell line stably expressing LTB, researchers fused the LTB coding sequence, neomycin-resistance gene (Neo(R)) and gfp gene into piggyBac-based fransponson vector and transduced into silkworm BmN cells. After screening against antibiotic G418, the positive rate of cells emitting green fluorescence was 70.79%. PCR detection indicates the existence of exogenous LTB coding sequence, Neo(R) and gfp in the transformed cell genome. Western blot analysis also confirms the predicated similar to 60 kDa band of LTB protein. These results demonstrated that the strategy was practicable.

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