Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms

文献类型： 外文期刊

第一作者： Cong, Liang

作者： Cong, Liang;Cao, Guangli;Xue Renyu;Pan Zhonghua;Zheng Xiaojian;Gong, Chengliang;Cao, Guangli;Xue Renyu;Pan Zhonghua;Gong, Chengliang;Zhou, Wenlin

作者机构：

关键词： Transgenic silkworms;hIGF-I;Silk glands;TIDM mice

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2011 年 410 卷 4 期

页码：

收录情况： SCI

摘要： To realize realize the secretory expression of human insulin-like growth factor-I (hIGF-I) in the posterior silk glands (PSGs) of transgenic silkworms, the piggyBac transposon vector pigA3GFP-fibHS-hIGF-i.e.-neo containing a neomycin-resistance gene (neo), green fluorescent protein gene (gfp) and human insulin-like growth factor I (hIGF-I) gene controlled by the Bombyx mori fibroin heavy chain gene (fib-H) promoter with its downstream signal peptide sequence, and a helper plasmid containing the piggyBac transposase sequence under the control of the B. mori actin 3 gene (A3) promoter were transferred into silkworm eggs by sperm-mediated gene transfer. Transformed silkworms were obtained after being screened for green fluorescence and by the antibiotic G418. In the PSGs of the transformed silkworms, a specific band representing hIGF-I could be detected by Western blotting, and the content of the hIGF-I estimated by ELISA was approximately 1.84 mu g/gram of cocoon and 19.18 mu g/gram of freeze-dried PSG powder. To further estimate the biological activity of the expressed hIGF-I, streptozotocin-induced TIDM mice were orally administered with the PSG powder of the transgenic silkworms, the results showed the blood glucose levels of mice were significantly reduced, suggesting that the the PSGs powder of transgenic hIGF-I silkworms could possibly be used as a perorally administered medicine. (C) 2011 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector. Wang, SP,Guo, TQ,Guo, XY,Huang, JT,Lu, CD.

[2]Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells. Li, Yanmei,Cao, Guangli,Wang, Yang,Xue, Renyu,Gong, Chengliang,Cao, Guangli,Xue, Renyu,Gong, Chengliang,Zhou, Wenlin,Gong, Chengliang.

作者其他论文 更多>>

微信小程序